Human Plexin A4 PE-conjugated Antibody

    
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  • Species Reactivity
    Human
  • Specificity
    Detects human Plexin A4 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Plexin A1, recombinant mouse (rm) Plexin A1 or rmPlexin A2 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 707206
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human Plexin A4
    Thr24-Pro1237
    Accession # Q9HCM2
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of Plexin A4 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3 epsilon APC‑conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human Plexin A4 PE‑conjugated Monoclonal Antibody (Catalog # FAB58561P) or (B) Mouse IgG1 Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Plexin A4

Plexin A4 is a 220‑230 kDa member of the plexin A subfamily, plexin family of proteins (1). It is found on sensory, autonomic and motor neurons and oligodendrocytes, plus T cells and dendritic cells (1‑8). Mature human Plexin A4 is an 1871 amino acid (aa) type I transmembrane glycoprotein with a 23 aa signal sequence, a 1214 aa extracellular domain (ECD), and a 636 aa cytoplasmic region. The ECD contains one Sema-domain (aa 51‑482), three PSI domains (aa 509‑856) and four IPT regions (aa 858‑1230) that contain a phosphoserine at aa 946 (1). Of three isoform variants, one shows a 65 aa substitution for aa 458‑1894, a second shows an 80 aa substitution for aa 1292‑1894, and a third shows the just mentioned 80 aa substitution coupled to a 14 aa substitution for aa 1‑535 (9). The human Plexin A4 ECD shares 97% aa identity with mouse, equine, canine, and bovine Plexin A4. Full‑length Plexin A4 also shares 67% aa identity with the most related family member, Plexin A2. Plexin A4 regulates cell migration, activation and axon guidance via repulsion (1‑5). It serves as a receptor for transmembrane semaphorins, Sema6A and 6B, and as a coreceptor with neuropilin-1 for the secreted semaphorin, Sema3A (1‑8). During development, it plays a role in nerve migration and midline crossing and down‑regulates dendrite formation (2‑8). It is often co‑expressed with Plexin A3, which can also engage class 6 semaphorins but prefers Sema3F/neuropilin‑2 to Sema3A/neuropilin-1 (3, 8). Thus, Plexins A3 and A4 are redundant in some functions, but unique in others. In T cells, Plexin A4 engages Sema3A and negatively regulates TCR signals (6).

  • References:
    1. Suto, F. et al. (2003) Mech. Dev. 120:385.
    2. Suto, F. et al. (2005) J. Neurosci. 25:3628.
    3. Faulkner, R.L. et al. (2008) Neural Dev. 3:21.
    4. Waimey, K.E. et al. (2008) Dev. Biol. 315:448.
    5. Runker, A.E. et al. (2008) Neural Dev. 3:34.
    6. Yamamoto, M. et al. (2008) Int. Immunol. 20:413.
    7. Okada, A. et al. (2007) Biochem. Biophys. Res. Commun. 352:158.
    8. Yaron, A. et al. (2005) Neuron 45:513.
    9. Protein Accession # NP_861440, EAW83796, EAL24077.
  • Entrez Gene IDs:
    91584 (Human); 243743 (Mouse); 312213 (Rat)
  • Alternate Names:
    A; DKFZp434G0625; FAYV2820; FLJ35026; FLJ38287; KIAA1550DKFZp566O0546; PLEXA4; Plexin A4; plexin A4, B; plexin-A4; PLXNA4; PLXNA4A; PLXNA4B; PRO34003
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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 PE-conjugated Antibody

Ctrl IC002P 39  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 7
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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