Human/Primate SLC6A3/DAT1 Extracellular Loop 2 Antibody

Name: Human/Primate SLC6A3/DAT1 Extracellular Loop 2 Antibody
Brand: R&D Systems
SKU: PPS069

R&D Systems | Catalog # PPS069

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Product Documents

Discontinued Product

PPS069 has been discontinued. View all SLC6A3/DAT1 products.

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Primate

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all SLC6A3/DAT1 Primary Antibodies »

Product Specifications

Immunogen

SLC6A3/DAT1

Specificity

Specific for the ~88 kDa DAT protein in Western blots of SDS-solubilized human striatal samples and in IHC applications withformaldehyde-fixed human and monkey brain sections.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human/Primate SLC6A3/DAT1 Extracellular Loop 2 Antibody

Detection of SLC6A3/DAT1 (Extracellular Loop 2) by Western Blot.

Western blot of SDS-solubilized human caudate (20 μg protein) showing the ~88 kDa band representing the DAT protein.

Applications for Human/Primate SLC6A3/DAT1 Extracellular Loop 2 Antibody

Application

Recommended Usage

Immunohistochemistry

1:1000 dilution
Sample: (frozen sections)

Western Blot

1:1000 dilution

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: SLC6A3/DAT1

The dopamine transporter (DAT) is a 70-80 kDa member of the Na⁺-neurotransmitter symporter family of transmembrane (TM) proteins. DAT plays a crucial role in the synaptic clearance of dopamine (DA). It mediates the uptake of dopamine by the presynaptic terminal, thus limiting the strength of the dopamingeric response. One molecule of DA is accompanied by two Na⁺ and one Cl^- ion. Molecules such as amphetamine both competitively inhibit DA uptake, and induce DA release through the DAT, increasing the rewarding property of DA. Human DAT is a 620 amino acid (aa), 12 TM phosphoglycoprotein with an N- and C-terminal cytoplasmic domain. It exists as a disulfide-linked oligomer on the cell surface. Phosphorylation of the N-terminus (S7/12) promotes DA release. The C-terminus binds CaMKII, as well as Hic5, Pick1, and synuclein which regulate receptor trafficking and expression. There is an extended 71 aa extracellular (EC) loop between TM segments 3 and 4. Glycosylation at this site is necessary for oligomer expression on the cell membrane. The human 42 aa DAT C-terminus is 93% aa identical to the mouse DAT
C-terminus. The human EC loop is also 93% aa identical to the rhesus monkey EC loop.

References

Torres, G.E. (2006) J. Neurochem. 97(Suppl. 1):3.
Sotnikova, T.D. et al. (2006) CNS Neurol. Disord. Drug Targets. 5:45.
Fog, J.U. et al. (2006) Neuron 51:417.
Giros, B. et al. (1992) Mol. Pharmacol. 42:383.
Cervinski, M.A. et al. (2005) J. Biol. Chem. 280:40442.

Long Name

Dopamine Transporter

Alternate Names

DAT1, SLC6A3, VNTR

Entrez Gene IDs

6531 (Human); 13162 (Mouse); 24898 (Rat)

Gene Symbol

SLC6A3

Additional SLC6A3/DAT1 Products

Product Documents for Human/Primate SLC6A3/DAT1 Extracellular Loop 2 Antibody

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Certificate of Analysis

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Product Specific Notices for Human/Primate SLC6A3/DAT1 Extracellular Loop 2 Antibody

For research use only

Related Research Areas

Neuronal Markers

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