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Human PRL/PTP4A Pan Specific Alexa Fluor® 532-conjugated Antibody

Catalog #: FAB32191X Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB32191X-100UG

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Human PRL/PTP4A Pan Specific Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human PRL-2 and human PRL-3 with equal affinities in direct ELISAs. In direct ELISAs, this antibody shows 10% cross-reactivity with recombinant human PRL-1.
Source
Monoclonal Mouse IgG2b Clone # 334407
Purification
Protein A or G purified
Immunogen
E. coli-derived recombinant human PRL-3
Ala2-Met173
Accession # O75365
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration
Sample
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: PRL/PTP4A

Phosphatase of Regenerating Liver (PRL), also known as PTP4A, dephosphorylates tyrosine residues on proteins. Three PRL variants, PRL-1 (PTP4A1), PRL-2 (PTP4A2), and PRL-3 (PTP4A3) have been cloned and are 75% to 86% homologous. Prenylation at the C-terminal end targets PRL family members to membrane compartments in the cell, especially to early endosomes and the plasma membrane. PRL levels may correlate with cell migration rates and tumor invasiveness, and PRL overexpression is known to cause an increase in the metastatic capability of cancer cell lines.

Long Name
Phosphatase of Regenerating Liver
Alternate Names
HH72;PRL1;PRL-1;Protein tyrosine phosphatase type IVA 1;PTP(CAAX1);PTP4A1;PTPCAAX1; PRL/PTP4A

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