Human PRL/PTP4A Pan Specific Antibody

Catalog # Availability Size / Price Qty
MAB32191
MAB32191-SP
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews

Human PRL/PTP4A Pan Specific Antibody Summary

Species Reactivity
Human
Specificity
Detects human PRL-2 and human PRL-3 with equal affinities in direct ELISAs. In direct ELISAs, this antibody shows 10% cross-reactivity with recombinant human PRL-1.
Source
Monoclonal Mouse IgG2B Clone # 334407
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human PRL-3
Ala2-Met173
Accession # O75365
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
Immersion fixed paraffin-embedded sections of human colon cancer tissue

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PRL/PTP4A

Phosphatase of Regenerating Liver (PRL), also known as PTP4A, dephosphorylates tyrosine residues on proteins. Three PRL variants, PRL-1 (PTP4A1), PRL-2 (PTP4A2), and PRL-3 (PTP4A3) have been cloned and are 75% to 86% homologous. Prenylation at the C-terminal end targets PRL family members to membrane compartments in the cell, especially to early endosomes and the plasma membrane. PRL levels may correlate with cell migration rates and tumor invasiveness, and PRL overexpression is known to cause an increase in the metastatic capability of cancer cell lines.

Long Name
Phosphatase of Regenerating Liver
Alternate Names
HH72;PRL1;PRL-1;Protein tyrosine phosphatase type IVA 1;PTP(CAAX1);PTP4A1;PTPCAAX1; PRL/PTP4A

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Citations for Human PRL/PTP4A Pan Specific Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Inhibiting MAPK14 showed anti-prolactinoma effect
    Authors: QY Ding, Y Zhang, L Ma, YG Chen, JH Wu, HF Zhang, X Wang
    BMC Endocr Disord, 2020;20(1):138.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Critical relevance of genomic gains of PRL-3/EGFR/c-myc pathway genes in liver metastasis of colorectal cancer
    Authors: T Tanaka, T Kaida, K Yokoi, S Ishii, N Nishizawa, H Kawamata, H Katoh, T Sato, T Nakamura, M Watanabe, K Yamashita
    Oncol Lett, 2019;17(1):1257-1266.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. Nomogram Incorporating CD44v6 and Clinicopathological Factors to Predict Lymph Node Metastasis for Early Gastric Cancer
    PLoS ONE, 2016;11(8):e0159424.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. Metastasis-associated phosphatase PRL-2 regulates tumor cell migration and invasion.
    Authors: Wang Y, Lazo JS,
    Oncogene, 2012;31(7):818-27.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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