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Human Pro-Collagen I alpha 1 Quantikine ELISA Kit

R&D Systems | Catalog # DPCA00

R&D Systems
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Key Product Details

Sample Type & Volume Required Per Well

Cell Culture Supernates (25 µL), Cell Lysates (25 µL), Tissue Lysates (25 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Saliva (10 µL), Urine (25 µL)

Sensitivity

8.75 pg/mL

Assay Range

78.1-5000 pg/mL (Cell Culture Supernates, Cell Lysates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
View all Collagen I alpha 1 ELISA Kits »

Product Summary for Human Pro-Collagen I alpha 1 Quantikine ELISA Kit

The Quantikine Human Pro-Collagen I alpha 1/COLIA1 (Pro-Collagen I alpha 1) Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human Pro-Collagen I alpha 1 in cell culture supernates, cell lysates, tissue extracts, serum, plasma, saliva, and urine. It contains recombinant human Pro-Collagen I alpha 1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Pro-Collagen I alpha 1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Pro-Collagen I alpha 1.

Product Specifications

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human Pro-Collagen I alpha 1. 

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.

Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Saliva, Serum, Tissue Lysates, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 442 1410 2669 448 1378 2677
Standard Deviation 17.8 32.4 50.1 28.9 49.8 109
CV% 4.0 2.3 1.9 6.4 3.6 4.1

Recovery for Human Pro-Collagen I alpha 1 Quantikine ELISA Kit

The recovery of human Pro-Collagen I alpha 1 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Extraction Buffer (n=1) 105 101-110
Lysis Buffer (n=1) 97 94-101
Media (n=4) 107 97-118
Saliva (n=4) 97 87-114
Urine (n=4) 90 79-111

Linearity

To assess the linearity of the assay, samples containing high concentrations of human Pro-Collagen I alpha 1 were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Images for Human Pro-Collagen I alpha 1 Quantikine ELISA Kit

Human Pro-Collagen I alpha 1 ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Collagen I alpha 1

Type I collagen is the most abundant structural protein of connective tissues such as skin, bone and tendon. It is synthesized as a procollagen molecule which is characterized by a 300 nm triple helical domain flanked by globular N- and C-terminal propeptides. The triple helical domain contains Gly-Xaa-Yaa triplets where Xaa and Yaa are frequently proline and hydroxyproline, respectively. The non-helical propeptides are removed by procollagen N- and C-proteinase activities so that the mature triple helices can self-assemble into collagen fibrils that provide tensile strength to tissues. Type I collagen is a heterotrimer that consists of two alpha 1(I) chains and one alpha 2(I) chain, although homotrimers consisting of three identical alpha 1(I) chains have also been described. This recombinant mini pro-alpha 1(I) collagen consists of a shortened alpha 1(I) chain with following domain structure from N- to C-terminus: N-propeptide, N-telopeptide, the 33 most N-terminal Gly-Xaa-Yaa repeats, the 33 most C-terminal Gly-Xaa-Yaa repeats, C-telopeptide and C-propeptide. The preparation contains a mixture of the full-length molecule, pN collagen I (alpha 1) and the C-terminal propeptide. This truncated pro-alpha 1(I) collagen is a substrate for procollagen N-proteinase and procollagen C-proteinase.

Alternate Names

COL1A1, OI4

Entrez Gene IDs

1277 (Human); 12842 (Mouse); 29393 (Rat)

Gene Symbol

COL1A1

Additional Collagen I alpha 1 Products

Product Documents for Human Pro-Collagen I alpha 1 Quantikine ELISA Kit

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Pro-Collagen I alpha 1 Quantikine ELISA Kit

For research use only

Citations for Human Pro-Collagen I alpha 1 Quantikine ELISA Kit

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