Human Pro-MMP-10 Quantikine ELISA Kit

(11 citations)   
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (25 uL), Heparin Plasma (25 uL)
  • Sensitivity
    15.1 pg/mL
  • Assay Range
    78.1 - 5,000 pg/mL (Cell Culture Supernates, Serum, Heparin Plasma)
  • Specificity
    Natural and recombinant human pro-MMP-10
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
Product Summary
The Quantikine Human Pro-MMP-10 Immunoassay is a 4.5 hour solid phase immunoassay designed to measure pro-MMP-10 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human pro-MMP-10 and antibodies raised against the recombinant protein. This kit will not detect human active MMP-10 existing in either free or TIMP-bound form. Natural human pro-MMP-10 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural human pro-MMP-10.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Standard Deviation22.911513730.4119153


The recovery of pro-MMP-10 spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 99 88-109
To assess the linearity of the assay, samples containing or spiked with high concentrations of pro-MMP-10 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Pro-MMP-10 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: MMP-10

The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).

    • Long Name
      Matrix Metalloproteinase 10
    • Entrez Gene IDs
      4319 (Human);
    • Alternate Names
      EC 3.4.24; EC; matrix metallopeptidase 10 (stromelysin 2); matrix metalloprotease 10; matrix metalloproteinase 10 (stromelysin 2); Matrix metalloproteinase-10; MMP10; MMP-10; SL-2; STMY2stromelysin-2; stromelysin 2; transin 2; transin-2;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 10 of 11
    Filter your results:

    Sample Type
    1. A multiplex immunoassay for the non-invasive detection of bladder cancer.
      Authors: Shimizu Y, Furuya H, Bryant Greenwood P, Chan O, Dai Y, Thornquist M, Goodison S, Rosser C
      J Transl Med, 2016;14(1):31.
      Species: Human
      Sample Type: Urine
    2. Suppression of Aurora-A-FLJ10540 signaling axis prohibits the malignant state of head and neck cancer.
      Authors: Chen, Chang-Ha, Chang, Alice Y, Li, Shau-Hsu, Tsai, Hsin-Tin, Shiu, Li-Yen, Su, Li-Jen, Wang, Wen-Lung, Chiu, Tai-Jen, Luo, Sheng-De, Huang, Tai-Lin, Chien, Chih-Yen
      Mol Cancer, 2015;14(0):83.
      Species: Human
      Sample Type: Cell Culture Supernates
    3. MMP-1 and Pro-MMP-10 as potential urinary pharmacodynamic biomarkers of FGFR3-targeted therapy in patients with bladder cancer.
      Authors: Du X, Lin B, Wang Q, Li H, Ingalla E, Tien J, Rooney I, Ashkenazi A, Penuel E, Qing J
      Clin Cancer Res, 2014;20(24):6324-35.
      Species: Human
      Sample Type: Serum
    4. Primary human acute myelogenous leukemia cells release matrix metalloproteases and their inhibitors: release profile and pharmacological modulation.
      Authors: Reikvam H, Hatfield KJ, Oyan AM, Kalland KH, Kittang AO, Bruserud O
      Eur. J. Haematol., 2010;84(3):239-51.
      Species: Human
      Sample Type: Cell Culture Supernates
    5. Matrix metalloproteinase-9, -10, and tissue inhibitor of matrix metalloproteinases-1 blood levels as biomarkers of severity and mortality in sepsis.
      Authors: Lorente L, Martin MM, Labarta L, Diaz C, Sole-Violan J, Blanquer J, Orbe J, Rodriguez JA, Jimenez A, Borreguero-Leon JM, Belmonte F, Medina JC, Lliminana MC, Ferrer-Aguero JM, Ferreres J, Mora ML, Lubillo S, Sanchez M, Barrios Y, Sierra A, Paramo JA
      Crit Care, 2009;13(5):R158.
      Species: Human
      Sample Type: Serum
    6. Advanced glycation end products decrease mesangial cell MMP-7: a role in matrix accumulation in diabetic nephropathy?
      Authors: McLennan SV, Kelly DJ, Schache M, Waltham M, Dy V, Langham RG, Yue DK, Gilbert RE
      Kidney Int., 2007;72(4):481-8.
      Species: Human
      Sample Type: Cell Culture Supernates
    7. C-reactive protein induces matrix metalloproteinase-1 and -10 in human endothelial cells: implications for clinical and subclinical atherosclerosis.
      Authors: Montero I, Orbe J, Varo N, Beloqui O, Monreal JI, Rodriguez JA, Diez J, Libby P, Paramo JA
      J. Am. Coll. Cardiol., 2006;47(7):1369-78.
      Species: Human
      Sample Type: Whole Cells
    8. Matrix metalloproteinase 10 promotion of collagenolysis via procollagenase activation: implications for cartilage degradation in arthritis.
      Authors: Barksby HE, Milner JM, Patterson AM, Peake NJ, Hui W, Robson T, Lakey R, Middleton J, Cawston TE, Richards CD, Rowan AD
      Arthritis Rheum., 2006;54(10):3244-53.
      Species: Human
      Sample Type: Synovial Fluid
    9. Induction of host matrix metalloproteinases by Borrelia burgdorferi differs in human and murine lyme arthritis.
      Authors: Behera AK, Hildebrand E, Scagliotti J, Steere AC, Hu LT
      Infect. Immun., 2005;73(1):126-34.
      Species: Human
      Sample Type: Synovial Fluid
    10. CXCL12-CXCR4 interactions modulate prostate cancer cell migration, metalloproteinase expression and invasion.
      Authors: Singh S, Singh UP, Grizzle WE, Lillard JW
      Lab. Invest., 2004;84(12):1666-76.
      Species: Human
      Sample Type: Cell Culture Supernates
    11. Induction of MMP-10 and MMP-1 in a squamous cell carcinoma cell line by ultraviolet radiation.
      Authors: Ramos MC, Steinbrenner H, Stuhlmann D, Sies H, Brenneisen P
      Biol. Chem., 2004;385(1):75-86.
      Species: Human
      Sample Type: Cell Culture Supernates
    Expand to show all
    ELISA Controls
    Description Application Cat# Citations Images  

    Quantikine Immunoassay Control Set 837 for Human Pro-MMP-10

    Ctrl QC131
    View Sizes & Prices
    Catalog# Size Price Stock Quantity
    Loading Information....
    Supplemental ELISA Products
    Description Application Cat# Citations Images  

    Quantikine Wash Buffer 1

    ELISA WA126 5
    View Sizes & Prices
    Catalog# Size Price Stock Quantity
    Loading Information....

    Have you used Human Pro-MMP-10 Quantikine ELISA Kit?

    Submit a review and receive a $25 amazon gift card if you include an image - $10 Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

    Order Details

    Contact Information

    Certificate of Analysis Lookup

    The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided

    Certificate of Analysis Lookup

    Research Areas of Interest
    R&D Systems Guarantee


    Customer Information