Natural and recombinant human Prolactin and natural human macroprolactin
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference observed with 1 or more available related molecules.
QC149, Quantikine Immunoassay Control Set 766 for Human Prolactin - Please Inquire
The Quantikine Human Prolactin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Prolactin in serum and plasma. It contains E. coli-expressed recombinant human Prolactin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Prolactin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Prolactin.
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in separate assays to
assess inter-assay precision. Assays were performed by at least three
technicians using two lots of kit components.
Serum, EDTA Plasma, Heparin Plasma
The recovery of Prolactin spiked to levels throughout the range of the assay was evaluated.
Average % Recovery
EDTA Plasma (n=4)
Heparin Plasma (n=4)
To assess the linearity of the assay, samples containing and/or spiked with high
concentrations of Prolactin were serially diluted with Calibrator Diluent
to produce samples with values within the dynamic range of the assay.
Preparation and Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Prolactin (PRL) is a neuroendocrine pituitary hormone that is synthesized by anterior pituitary, placenta, brain, uterus, dermal fibroblasts, decidua, B cells, T cells, natural killer cells, and breast cancer cells. The prolactin receptor is a transmembrane type I glycoprotein that belongs to the hematopoietic cytokine receptor family.
Prolactin receptor (PRL R) contains an extracellular, transmembrane, and intracellular domain. Transcriptional regulation of the PRL R gene results in several different species-dependent isoforms of PRL R being produced.