Natural and recombinant human Prolactin and natural human macroprolactin
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference observed with 1 or more available related molecules.
QC149 , Quantikine Immunoassay Control Set 766 for Human Prolactin -
The Quantikine Human Prolactin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Prolactin in serum and plasma. It contains E. coli-expressed recombinant human Prolactin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Prolactin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Prolactin.
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in separate assays to
assess inter-assay precision. Assays were performed by at least three
technicians using two lots of kit components
Serum, EDTA Plasma, Heparin Plasma
The recovery of Prolactin spiked to levels throughout the range of the assay was evaluated.
Average % Recovery
EDTA Plasma (n=4)
Heparin Plasma (n=4)
To assess the linearity of the assay, samples containing and/or spiked with high
concentrations of Prolactin were serially diluted with Calibrator Diluent
to produce samples with values within the dynamic range of the assay.
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Prolactin (PRL) is a neuroendocrine pituitary hormone that is synthesized by anterior pituitary, placenta, brain, uterus, dermal fibroblasts, decidua, B cells, T cells, natural killer cells, and breast cancer cells. The prolactin receptor is a transmembrane type I glycoprotein that belongs to the hematopoietic cytokine receptor family.
Prolactin receptor (PRL R) contains an extracellular, transmembrane, and intracellular domain. Transcriptional regulation of the PRL R gene results in several different species-dependent isoforms of PRL R being produced.
Refer to the product for complete assay procedure.
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
Prepare all reagents, standard dilutions, and samples as directed in the product insert.
Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
100 µL Assay Diluent
Add 100 µL of Assay Diluent to each well.
50 µL Standard, Control, or Sample
Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
200 µL Conjugate
Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
Aspirate and wash 4 times.
200 µL Substrate Solution
Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
50 µL Stop Solution
Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 -
First-in-human Clinical Trial of Oral ONC201 in Patients with Refractory Solid Tumors
Authors: MN Stein, JR Bertino, HL Kaufman, T Mayer, R Moss, A Silk, N Chan, J Malhotra, L Rodriguez-, J Aisner, RD Aiken, BG Haffty, RS DiPaola, T Saunders, A Zloza, S Damare, Y Beckett, B Yu, S Najmi, C Gabel, S Dickerson, L Zheng, WS El-Deiry, JE Allen, M Stogniew, W Oster, JM Mehnert
Clin. Cancer Res, 2017;0(0):.
Sample Type: Serum
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