Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Sheep IgG
View all Prostasin/Prss8 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Prostasin/Prss8
Ala33-Gly319
Accession # Q16651

Specificity

Detects human Prostasin/Prss8 in Western blots. In Western blots, approximately 10% cross‑reactivity with recombinant mouse Prostasin is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human Prostasin/Prss8 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Prostasin/Prss8 (Catalog # 4599-SE)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Prostasin/Prss8

Prostasin, also known as channel activating protease 1, is a serine protease with trypsin-like substrate specificity (1, 2). The preproenzyme possesses a C-terminal membrane-spanning domain that can be proteolytically processed to generate a secreted form of the enzyme. The secreted form of Prostasin has been purified from seminal fluid (2). Prostasin is highly expressed in the prostate gland, and is expressed at lower levels in the lung, kidney, liver, salivary gland, and pancreas (3). The peptidase activity of Prostasin is involved in the regulation of epithelial sodium channels (4, 5).

References

  1. Vuagniaux, G. et al. (2000) J. Am. Soc. Nephrol. 11:828.
  2. Yu, J.X. et al. (1994) J. Biol. Chem. 269:18843.
  3. Yu, J.X. et al. (1995) J. Biol. Chem. 270:13483.
  4. Caldwell, R.A. et al. (2004) Am. J. Physiol. Cell Physiol. 286:C194.
  5. Tong, Z. et al. (2004) Am. J. Physiol. Lung Cell Mol. Physiol. 287:L928.

Alternate Names

Prss8

Entrez Gene IDs

5652 (Human); 76560 (Mouse)

Gene Symbol

PRSS8

UniProt

Q16651

Additional Prostasin/Prss8 Products

Product Documents for Human Prostasin/Prss8 Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Prostasin/Prss8 Biotinylated Antibody

For research use only

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