Ras-related protein Rab25, also known as CATX-8, is a 23 kDa (unglycosylated) member of the small GTPase superfamily and Rab family of proteins. Human Rab25 is 213 amino acids (aa) in length. The mature chain runs from aa 1-210 with residues 211-213 constituting a propeptide. Residues 10-174 are noted to be Rab11-like. Rab11a, Rab11b, and Rab25 are closely related Rab proteins that are differentially expressed. Human Rab25 shares 96% aa sequence identity with mouse Rab25. Functionally, Rab25 is involved in the regulation of cell survival. Researchers have shown that Rab25 promotes invasive migration of cells and increases the rates and aggressiveness of breast and ovarian cancers. It is expressed in breast and ovarian tissues.
Human RAB25 Alexa Fluor™ Plus 647‑conjugated Antibody
R&D Systems | Catalog # AF6605AFP647
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Alexa Fluor Plus 647 (Excitation = 658 nm, Emission = 675 nm)
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Specificity
Detects human RAB25 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human RAB25 Alexa Fluor™ Plus 647‑conjugated Antibody
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Rab25
Long Name
RAs Genes from Brain Protein 25
Alternate Names
CATX8, RAB11C
Gene Symbol
RAB25
UniProt
Additional Rab25 Products
Product Documents for Human RAB25 Alexa Fluor™ Plus 647‑conjugated Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human RAB25 Alexa Fluor™ Plus 647‑conjugated Antibody
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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