Human RAB25 Antibody Summary
Accession # P57735
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human RAB25 by Western Blot. Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, and SK-BR-3 human breast cancer cell line. PVDF Membrane was probed with 2 µg/mL of Human RAB25 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6605) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for RAB25 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Ras-related protein Rab25, also known as CATX-8, is a 23 kDa (unglycosylated) member of the small GTPase superfamily and Rab family of proteins. Human Rab25 is 213 amino acids (aa) in length. The mature chain runs from aa 1-210 with residues 211-213 constituting a propeptide. Residues 10-174 are noted to be Rab11-like. Rab11a, Rab11b, and Rab25 are closely related Rab proteins that are differentially expressed. Human Rab25 shares 96% aa sequence identity with mouse Rab25. Functionally, Rab25 is involved in the regulation of cell survival. Researchers have shown that Rab25 promotes invasive migration of cells and increases the rates and aggressiveness of breast and ovarian cancers. It is expressed in breast and ovarian tissues.
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