DOCK1 (Dedicator of cytokinesis protein 1) is a member of the DOCK-A family, DOCK180 superfamily of proteins. Although its predicted molecular weight is 215 kDa, it runs anomalously at 180-190 kDa in SDS-PAGE. It is generally found in non-hematopoietic cell types, and transduces signals from cell membrane receptors by regulating the activity of the GTPases Rac-1 and -2. This impacts both cell migration and phagocytosis. Human DOCK1 is 1865 amino acids (aa) in length. It contains one protein-interaction SH3 domain (aa 9-70), a DHR-1 region that localizes DOCK1 to the plasma membrane and PI3 kinase (aa 422-664), a DHR-2 domain that binds to nucleotide-free GTPases and shows GEF activity (aa 1111-1616), and two SH3-binding motifs (aa 1799-1843). There are four potential phosphorylation sites and one isoform variant that shows a start site at Met114. Over aa 422-666, human DOCK1 shares 97% aa identity with mouse DOCK1.
Human/Rat DOCK1 Alexa Fluor® 488‑conjugated Antibody
R&D Systems | Catalog # AF7070G
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Gly422-Ser666
Accession # Q14185
Specificity
Clonality
Host
Isotype
Applications for Human/Rat DOCK1 Alexa Fluor® 488‑conjugated Antibody
Western Blot
Formulation, Preparation, and Storage
Purification
Formulation
Shipping
Stability & Storage
Background: DOCK1
Long Name
Alternate Names
Gene Symbol
UniProt
Additional DOCK1 Products
Product Documents for Human/Rat DOCK1 Alexa Fluor® 488‑conjugated Antibody
Certificate of Analysis
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Product Specific Notices for Human/Rat DOCK1 Alexa Fluor® 488‑conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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Protocols
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- Western Blot Troubleshooting Guide
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