Human/Rat DOCK1 Antibody

• Species Reactivity
  Human, Rat
• Specificity
  Detects recombinant human DOCK1 in direct ELISAs. Detects human and rat DOCK1 in Western blots.
• Source
  Polyclonal Goat IgG
• Purification
  Antigen Affinity-purified
• Immunogen
  E. coli-derived recombinant human DOCK1
  Gly422-Ser666
  Accession # Q14185
• Formulation
  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Label
  Unconjugated

• Western Blot
  1 µg/mL
  See below

Western Blot click image to view larger

Detection of Human and Rat DOCK1 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells, HepG2 human hepatocellular carcinoma cell line, C6 rat glioma cell line, and PC‑12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human DOCK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7070) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for DOCK1 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reconstitution
  Sterile PBS to a final concentration of 0.2 mg/mL.

  Reconstitution Buffer Available
• Shipping
  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
• Stability & Storage
  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

• Long Name:
  Dedicator of Cytokinesis Protein 1

• Entrez Gene IDs:
  1793 (Human); 330662 (Mouse); 309081 (Rat)

• Alternate Names:
  180 kDa protein downstream of CRK; ced5; dedicator of cytokinesis 1; dedicator of cyto-kinesis 1; DOCK1; DOCK180; DOCK180dedicator of cytokinesis protein 1; DOwnstream of CrK

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