Human/Rat DOCK1 Antibody Summary
Accession # Q14185
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human and Rat DOCK1 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells, HepG2 human hepatocellular carcinoma cell line, C6 rat glioma cell line, and PC‑12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human DOCK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7070) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for DOCK1 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
DOCK1 (Dedicator of cytokinesis protein 1) is a member of the DOCK-A family, DOCK180 superfamily of proteins. Although its predicted molecular weight is 215 kDa, it runs anomalously at 180-190 kDa in SDS-PAGE. It is generally found in non-hematopoietic cell types, and transduces signals from cell membrane receptors by regulating the activity of the GTPases Rac-1 and -2. This impacts both cell migration and phagocytosis. Human DOCK1 is 1865 amino acids (aa) in length. It contains one protein-interaction SH3 domain (aa 9-70), a DHR-1 region that localizes DOCK1 to the plasma membrane and PI3 kinase (aa 422-664), a DHR-2 domain that binds to nucleotide-free GTPases and shows GEF activity (aa 1111-1616), and two SH3-binding motifs (aa 1799-1843). There are four potential phosphorylation sites and one isoform variant that shows a start site at Met114. Over aa 422-666, human DOCK1 shares 97% aa identity with mouse DOCK1.
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