phospho-5-Lipoxygenase (S523) Antibody

R&D Systems | Catalog # PPS001

R&D Systems
Discontinued Product
PPS001 has been discontinued. View all 5-Lipoxygenase products.

Key Product Details

Species Reactivity

Validated:

Human, Rat, Primate

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all 5-Lipoxygenase Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-S523 of human 5-Lipoxygenase (5-LO)

Specificity

Human and rat 5-Lipoxygenase phosphorylated at S523 in Western blots.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for phospho-5-Lipoxygenase (S523) Antibody

Detection of Phospho-5-Lipoxygenase (S523) antibody by Western Blot.

Detection of Phospho-5‑Lipoxygenase (S523) by Western Blot.

Western blot of rat brain (cortex) tissue lysate showing specific immunolabeling of the ~80 kDa doublet of 5-LO phosphorylated at S523 (Control). The phosphospecificity of this labeling is demonstrated by treatment with 1200 U of lambda Phosphatase ( lambda -PPase) for 30 minutes before being exposed to the Anti-Phospho-5-Lipoxygenase (S523). The immunolabeling is completely eliminated by treatment with lambda -PPase.

Applications for phospho-5-Lipoxygenase (S523) Antibody

Application
Recommended Usage

Western Blot

1:1000 dilution
Sample: Rat brain (cortex) tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: 5-Lipoxygenase

5-Lipoxygenase (5-LO) is an 80 kDa, 676 amino acid (aa) nonheme iron dioxygenase. It converts nuclear membrane arachidonic acid into leukotriene A4 (LTA4) that is then modified to produce either LTB4 or LTC4. 5-LO is found in leukocytes and generally exists in the cytoplasm. Upon activation, 5-LO will either remain cytosolic or undergo translocation into the nucleus. In the nucleus, its enzyme products impact gene expression; in the cytosol, its products are secreted and contribute to inflammation. 5-LO has three Nuclear Localization Signal (NLS) motifs starting at amino acids 112, 158, and 518. Upon cell activation, Ser at 523 is phosphorylated by Protein Kinase A (PKA). This amino acid lies within the principal NLS of 5-LO. When phosphorylated, the NLS is inactivated, blocking 5-LO entry into the nucleus. This appears to impact overall leukotriene biosynthesis, and potentially serves as a regulatory mechanism for inflammation.

References

  1. Jones, S.M. et al. (2003) J. Biol. Chem. 278:10257.
  2. Dixon, R.A. et al. (1988) Proc. Natl. Acad. Sci. USA 85:416.
  3. Luo, M. et al. (2005) J. Biol. Chem. 280:40609.
  4. Funk, C. (2001) Science 294:1871.

Alternate Names

5Lipoxygenase, 5LPG, ALOX5, LOG5, LOX5A

Entrez Gene IDs

240 (Human); 11689 (Mouse); 25290 (Rat)

Gene Symbol

ALOX5

Additional 5-Lipoxygenase Products

Product Documents for phospho-5-Lipoxygenase (S523) Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for phospho-5-Lipoxygenase (S523) Antibody

For research use only

Citations for phospho-5-Lipoxygenase (S523) Antibody

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