Human ROR gamma /RORC/NR1F3 PerCP‑conjugated Antibody

R&D Systems | Catalog # IC6109C

R&D Systems
Discontinued Product
IC6109C has been discontinued. View all ROR gamma/RORC/NR1F3 products.

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Flow Cytometry

Label

PerCP (Excitation = 482 and 564 nm, Emission = 675 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 600214
View all ROR gamma/RORC/NR1F3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ROR gamma /RORC/NR1F3
Met1-Gln100
Accession # P51449-2

Specificity

Detects human and mouse ROR gamma /RORC/NR1F3 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human ROR gamma /RORC/NR1F3 PerCP‑conjugated Antibody

Detection of ROR?/RORC/NR1F3 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of ROR gamma /RORC/NR1F3 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with PMA, Calcium Ionomycin, LPS, Recombinant Human IL-6 (Catalog # 206-IL) and Recombinant Human IL-23 (Catalog # 1290-IL) were stained with Mouse Anti-Human ROR gamma/RORC/NR1F3 PerCP-conjugated Monoclonal Antibody (Catalog # IC6109C) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # IC0041C). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human ROR gamma /RORC/NR1F3 PerCP‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with PMA, Calcium Ionomycin, LPS, Recombinant Human IL-6 (Catalog # 206-IL), and Recombinant Human IL‑23 (Catalog # 1290-IL) were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: ROR gamma/RORC/NR1F3

ROR gamma (Nuclear receptor ROR-gamma), also called RORC and NR1F3, is a member of the NR1 nuclear hormone receptor family. ROR gamma is a DNA binding transcription factor. ROR gamma is 518 amino acids (aa) in length. Deletion in mice implicates ROR gamma as being essential for lymphoid organogenesis and controlling apoptosis during thymopoiesis. Two splice forms differing in the first 24 aa have been found for this gene; isoform 2 deletes aa 1‑21 resulting in an alteration of aa 22‑24. Over aa 1‑100 human RORC shares 96% identity with mouse ROR gamma.

Long Name

Retinoic Acid-Related Orphan Receptor gamma

Alternate Names

NR1F3, RORC, RZRG

Entrez Gene IDs

6097 (Human)

Gene Symbol

RORC

UniProt

P51449-2

Additional ROR gamma/RORC/NR1F3 Products

Product Documents for Human ROR gamma /RORC/NR1F3 PerCP‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human ROR gamma /RORC/NR1F3 PerCP‑conjugated Antibody

For research use only

Citations for Human ROR gamma /RORC/NR1F3 PerCP‑conjugated Antibody

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FAQs for Human ROR gamma /RORC/NR1F3 PerCP‑conjugated Antibody

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  • Q: What is the immunogen sequence of this ROR gamma/RORC/NR1F3 antibody?

    A: A sequence from Met1 - Gln100.

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