Recombinant Human IL-23 Protein

Carrier Free

Catalog # Availability Size / Price Qty
1290-IL-010/CF
1290-IL-500/CF

With Carrier

Catalog # Availability Size / Price Qty
1290-IL-010
Recombinant Human IL-23 Protein Bioactivity
1 Image
Product Details
Citations (93)
FAQs
Reviews (6)

Recombinant Human IL-23 Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce IL-17 secretion by mouse splenocytes. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910. The ED50 for this effect is 0.05-0.3 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human IL-23 protein
Human IL-23 p40
(Ile23-Ser328)
Accession # P29460
GSGSSRGGSGSGGSGGGGSKL Human IL-23 p19
(Arg20-Pro189)
Accession # AAG37232
N-terminus C-terminus
N-terminal Sequence
Analysis
Ile23 (IL-23 single chain & p40) & Ala21 (p19)
Predicted Molecular Mass
55.0 kDa (IL-23 single chain); 36.5 kDa (p40) & 18.5 kDa (p19)
SDS-PAGE
65 kDa, 43-45 kDa and 20 kDa, reducing conditions

Product Datasheets

1290-IL (with carrier)

1290-IL/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1290-IL

Formulation Lyophilized from a 0.2 μm filtered solution in MES, NaCl and EDTA with BSA as a carrier protein.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1290-IL/CF

Formulation Supplied as a 0.2 μm filtered solution in MES, NaCl and EDTA.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Data Image

Bioactivity Recombinant Human IL-23 Protein Bioactivity View Larger

Recombinant Human IL-23 (Catalog # 1290-IL) induces IL-17 secretion by mouse splenocytes. The ED50 is approximately 2-fold better than top competitor. The protein activity is significantly better with nearly twice the max than the top competitor's IL-23.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-23

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1-5). The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. Human p19 cDNA encodes a 189 amino acid residue (aa) precursor protein with a putative 19 aa signal peptide and 170 aa mature protein. Human and mouse p19 share 70% aa sequence identity. Although p19 is expressed by activated macrophages, dendritic cells, T cells, and endothelial cells, only activated macrophages and dendritic cells express p40 concurrently to produce IL-23. The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R). IL-23 has biological activities that are similar to, but distinct from IL-12. Both IL-12 and IL-23 induce proliferation and IFN-gamma production by human T cells. While IL-12 acts on both naïve and memory human T cells, the effects of IL-23 is restricted to memory T cells. In mouse, IL-23 but not IL-12, has also been shown to induce memory T cells to secret IL-17, a potent proinflammatory cytokine. IL-12 and IL-23 can induce IL-12 production from mouse splenic DC of both the CD8- and CD8+ subtypes, however only IL-23 can act directly on CD8+ DC to mediate immunogenic presentation of poorly immunogenic tumor/self peptide.

References
  1. Oppmann, B. et al. (2000) Immunity 13:715.
  2. Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.
  3. Parham, C. et al. (2002) J. Immunol. 168:5699.
  4. Belladonna, M.L. et al. (2002) J. Immunol. 168:5448.
  5. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.
Long Name
Interleukin 23
Entrez Gene IDs
51561 (Human); 83430 (Mouse); 155140 (Rat); 102128555 (Cynomolgus Monkey)
Alternate Names
IL-23 p19/IL-12 p40; IL23; IL-23; IL-23A; IL-23-A; IL-23p19; IL-23p19/IL-12p40; IL23P19P19; interleukin 23 p19 subunit; interleukin 23, alpha subunit p19; interleukin-23 subunit alpha; Interleukin-23 subunit p19; JKA3 induced upon T-cell activation; MGC79388; SGRF; SGRFIL-23 subunit alpha

Citations for Recombinant Human IL-23 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

93 Citations: Showing 1 - 10
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  1. Myeloid-derived suppressor cell depletion therapy targets IL-17A-expressing mammary carcinomas
    Authors: B Dawod, J Liu, S Gebremeske, C Yan, A Sappong, B Johnston, DW Hoskin, JS Marshall, J Wang
    Sci Rep, 2020;10(1):13343.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Identification of two major autoantigens negatively regulating endothelial activation in Takayasu arteritis
    Authors: T Mutoh, T Shirai, T Ishii, Y Shirota, F Fujishima, F Takahashi, Y Kakuta, Y Kanazawa, A Masamune, Y Saiki, H Harigae, H Fujii
    Nat Commun, 2020;11(1):1253.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  3. Recapitulating T cell infiltration in 3D psoriatic skin models for patient-specific drug testing
    Authors: JU Shin, HE Abaci, L Herron, Z Guo, B Sallee, A Pappalardo, J Jackow, EHC Wang, Y Doucet, AM Christiano
    Sci Rep, 2020;10(1):4123.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  4. Cytokines regulate the antigen-presenting characteristics of human circulating and tissue-resident intestinal ILCs
    Authors: A Rao, O Strauss, E Kokkinou, M Bruchard, KP Tripathi, H Schlums, A Carrasco, L Mazzurana, V Konya, EJ Villablanc, NK Björkström, U Lindforss, H Spits, J Mjösberg
    Nat Commun, 2020;11(1):2049.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Prolactin Acts on Myeloid Progenitors to Modulate SMAD7 Expression and Enhance Hematopoietic Stem Cell Differentiation into the NK Cell Lineage
    Authors: DM Tufa, T Shank, AM Yingst, GD Trahan, S Shim, J Lake, R Woods, K Jones, MR Verneris
    Sci Rep, 2020;10(1):6335.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Effects of resveratrol on Th17 cell-related immune responses under tacrolimus-based immunosuppression
    Authors: KC Doh, BM Kim, KW Kim, BH Chung, CW Yang
    BMC Complement Altern Med, 2019;19(1):54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Expression of c-Kit discriminates between two functionally distinct subsets of human type 2 innate lymphoid cells
    Authors: T Hochdörfer, C Winkler, K Pardali, J Mjösberg
    Eur. J. Immunol., 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Two distinct colonic CD14+ subsets characterized by single-cell RNA profiling in Crohn's disease
    Authors: L Chapuy, M Bsat, S Sarkizova, M Rubio, A Therrien, E Wassef, M Bouin, K Orlicka, A Weber, N Hacohen, AC Villani, M Sarfati
    Mucosal Immunol, 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. cIAP1/2 inhibition synergizes with TNF inhibition in autoimmunity by down-regulating IL-17A and inducing Tregs
    Authors: JZ Kawalkowsk, J Ogbechi, PJ Venables, RO Williams
    Sci Adv, 2019;5(5):eaaw5422.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. c-Kit-positive ILC2s exhibit an ILC3-like signature that may contribute to IL-17-mediated pathologies
    Authors: JH Bernink, Y Ohne, MBM Teunissen, J Wang, J Wu, L Krabbendam, C Guntermann, R Volckmann, J Koster, S van Tol, I Ramirez, Y Shrestha, MA de Rie, H Spits, X Romero Ros, AA Humbles
    Nat. Immunol., 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  11. Data on arsenic trioxide modulates Treg/Th17/Th1/Th2 cells in treatment-na�ve rheumatoid arthritis patients and collagen-induced arthritis model mice
    Authors: C Li, J Zhang, W Wang, H Wang, Y Zhang, Z Zhang
    Data Brief, 2019;27(0):104615.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. The neuropeptide VIP confers anticipatory mucosal immunity by regulating ILC3 activity
    Authors: C Seillet, K Luong, J Tellier, N Jacquelot, RD Shen, P Hickey, VC Wimmer, L Whitehead, K Rogers, GK Smyth, AL Garnham, ME Ritchie, GT Belz
    Nat. Immunol., 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  13. Pathogenic function of bystander-activated memory-like CD4+ T cells in autoimmune encephalomyelitis
    Authors: HG Lee, JU Lee, DH Kim, S Lim, I Kang, JM Choi
    Nat Commun, 2019;10(1):709.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Increased Interleukin-23 in Hashimoto's Thyroiditis Disease Induces Autophagy Suppression and Reactive Oxygen Species Accumulation
    Authors: T Zheng, C Xu, C Mao, X Mou, F Wu, X Wang, L Bu, Y Zhou, X Luo, Q Lu, H Liu, G Yuan, S Wang, D Chen, Y Xiao
    Front Immunol, 2018;9(0):96.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. DDIT4 and Associated lncDDIT4 Modulate Th17 Differentiation through the DDIT4/TSC/mTOR Pathway
    Authors: F Zhang, G Liu, D Li, C Wei, J Hao
    J. Immunol., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Interleukin-23 promotes the migration and invasion of gastric cancer cells by inducing epithelial-to-mesenchymal transition via the STAT3 pathway
    Authors: X Xu, C Yang, J Chen, J Liu, P Li, Y Shi, P Yu
    Biochem. Biophys. Res. Commun., 2018;0(0):.
  17. A Rapamycin-Based GMP-Compatible Process for the Isolation and Expansion of Regulatory T Cells for Clinical Trials
    Authors: H Fraser, N Safinia, N Grageda, S Thirkell, K Lowe, LJ Fry, C Scottá, A Hope, C Fisher, R Hilton, D Game, P Harden, A Bushell, K Wood, RI Lechler, G Lombardi
    Mol Ther Methods Clin Dev, 2018;8(0):198-209.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Aberrant expression of NKL homeobox gene HLX in Hodgkin lymphoma
    Authors: S Nagel, C Pommerenke, C Meyer, M Kaufmann, RAF MacLeod, HG Drexler
    Oncotarget, 2018;9(18):14338-14353.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. IL-23 and IL-1? Drive Human Th17 Cell Differentiation and Metabolic Reprogramming in Absence of CD28 Costimulation
    Authors: S Revu, J Wu, M Henkel, N Rittenhous, A Menk, GM Delgoffe, AC Poholek, MJ McGeachy
    Cell Rep, 2018;22(10):2642-2653.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  20. The transcriptional coactivator TAZ regulates reciprocal differentiation of TH17 cells and Treg cells
    Authors: J Geng, S Yu, H Zhao, X Sun, X Li, P Wang, X Xiong, L Hong, C Xie, J Gao, Y Shi, J Peng, RL Johnson, N Xiao, L Lu, J Han, D Zhou, L Chen
    Nat. Immunol., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  21. TLR7 Signaling Regulates Th17 Cells and Autoimmunity: Novel Potential for Autoimmune Therapy
    Authors: J Ye, Y Wang, X Liu, L Li, A Opejin, EC Hsueh, H Luo, T Wang, D Hawiger, G Peng
    J. Immunol., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  22. An activated Th17-prone T cell subset involved in chronic graft-versus-host disease sensitive to pharmacological inhibition
    Authors: E Forcade, K Paz, R Flynn, B Griesenaue, T Amet, W Li, L Liu, G Bakoyannis, D Jiang, HW Chu, M Lobera, J Yang, DS Wilkes, J Du, K Gartlan, GR Hill, KP MacDonald, EL Espada, P Blanco, JS Serody, J Koreth, CS Cutler, JH Antin, RJ Soiffer, J Ritz, S Paczesny, BR Blazar
    JCI Insight, 2017;2(12):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Differentiation stage-specific effect of histone deacetylase inhibitors on the expression of ROR?T in human lymphocytes
    Authors: A Sa?kowska, K Kara?, A Walczak-Dr, J Dastych, M Ratajewski
    J. Leukoc. Biol., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  24. Rheumatoid arthritis bone marrow environment supports Th17 response
    Authors: E Kuca-Warna, W Kurowska, M Prochorec-, A Radzikowsk, T Burakowski, U Skalska, M Massalska, M Pleba?czyk, B Ma?dyk-Now, I S?owi?ska, R Gasik, W Ma?li?ski
    Arthritis Res. Ther., 2017;19(1):274.
    Species: Human
    Sample Types: Plasma
  25. PD-L1 up-regulation restrains Th17 cell differentiation in STAT3 loss- and STAT1 gain-of-function patients.
    Authors: Yuan Zhang, Chi A Ma, Monica G Lawrence, Timothy J Break, Michael P O'connell, Jonathan J Lyons, Diego B López, John S Barber, Yongge Zhao, Daniel L Barber, Alexandra F Freeman, Steven M Holland, Michail S Lionakis, Joshua D Milner
    The Journal of Experimental Medicine, 2017;0(0):1540-9538.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  26. Cross-Tissue Transcriptomic Analysis of Human Secondary Lymphoid Organ-Residing ILC3s Reveals a Quiescent State in the Absence of Inflammation
    Authors: YE Bar-Ephrai, F Cornelisse, N Papazian, T Konijn, RM Hoogenboez, MA Sanders, BA Westerman, M Gönültas, J Kwekkeboom, JMM Den Haan, RM Reijmers, RE Mebius, T Cupedo
    Cell Rep, 2017;21(3):823-833.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  27. The Stimulation of Macrophages with TLR Ligands Supports Increased IL-19 Expression in Inflammatory Bowel Disease Patients and in Colitis Models
    Authors: A Steinert, I Linas, B Kaya, M Ibrahim, A Schlitzer, P Hruz, K Radulovic, L Terraccian, AJ Macpherson, JH Niess
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  28. Neuropilin-1 Is Expressed on Lymphoid Tissue Residing LTi-like Group 3 Innate Lymphoid Cells and Associated with Ectopic Lymphoid Aggregates
    Authors: MM Shikhagaie, ÅK Björklund, J Mjösberg, JS Erjefält, AS Cornelisse, XR Ros, SM Bal, JJ Koning, RE Mebius, M Mori, M Bruchard, B Blom, H Spits
    Cell Rep, 2017;18(7):1761-1773.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  29. Preliminary study of histamine H4 receptor expressed on human CD4(+) T cells and its immunomodulatory potency in the IL-17 pathway of psoriasis
    Authors: SH Han, MS Hur, MJ Kim, BM Kim, KW Kim, HR Kim, YB Choe, KJ Ahn, YW Lee
    J. Dermatol. Sci., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  30. IL-33, IL-25, and TSLP induce a distinct phenotypic and activation profile in human type 2 innate lymphoid cells
    Authors: A Camelo, G Rosignoli, Y Ohne, RA Stewart, C Overed-Say, MA Sleeman, RD May
    Blood Adv, 2017;1(10):577-589.
    Species: Human
    Sample Types: Whole Cells
  31. A functional DC cross talk promotes human ILC homeostasis in humanized mice
    Authors: S Lopez-Last, G Masse-Rans, O Fiquet, S Darche, N Serafini, Y Li, M Dusséaux, H Strick-Mar, JP Di Santo
    Blood Adv, 2017;1(10):601-614.
    Species: Human
    Sample Types: Whole Cells
  32. Rheumatoid arthritis bone marrow environment supports Th17 response.
    Authors: Ewa Kuca-Warna, Weronika Kurowska, Monika Prochorec, Anna Radzikows, Tomasz Burakowsk, Urszula Skalska, Magdalena Massalska, Magdalena Pleba?czy, Barbara Ma?dyk-No, Iwona S?owi?ska, Robert Gasik, W?odzimierz Ma?li?ski
    Arthritis Research & Therapy, 2017;0(0):1478-6362.
    Species: Human
    Sample Types:
    Applications: ELISA (Standard)
  33. Developing in vitro expanded CD45RA+ regulatory T cells as an adoptive cell therapy for Crohn's disease
    Authors: James B Canavan
    Gut, 2016;65(4):584-94.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  34. Analysis of CXCR5(+)Th17 cells in relation to disease activity and TNF inhibitor therapy in Rheumatoid Arthritis
    Sci Rep, 2016;6(0):39474.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  35. Innate lymphoid cells are expanded and functionally altered in chronic lymphocytic leukemia
    Authors: I de Weerdt, V van Hoeven, JM Munneke, S Endstra, T Hofland, MD Hazenberg, AP Kater
    Haematologica, 2016;101(11):e461-e464.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  36. Human Mesenchymal Stem Cells Impact Th17 and Th1 Responses Through a Prostaglandin E2 and Myeloid-Dependent Mechanism
    Stem Cells Transl Med, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  37. Expression of human gene coding ROR?T receptor depends on the Sp2 transcription factor
    Authors: Marcin Ratajewski
    J Leukoc Biol, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  38. Pharmacologic modulation of ROR?t translates to efficacy in preclinical and translational models of psoriasis and inflammatory arthritis
    Sci Rep, 2016;6(0):37977.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  39. IL-34- and M-CSF-induced macrophages switch memory T cells into Th17 cells via membrane IL-1alpha.
    Authors: Foucher E, Blanchard S, Preisser L, Descamps P, Ifrah N, Delneste Y, Jeannin P
    Eur J Immunol, 2015;45(4):1092-102.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  40. Inherited IL-17RC deficiency in patients with chronic mucocutaneous candidiasis.
    Authors: Ling Y, Cypowyj S, Aytekin C, Galicchio M, Camcioglu Y, Nepesov S, Ikinciogullari A, Dogu F, Belkadi A, Levy R, Migaud M, Boisson B, Bolze A, Itan Y, Goudin N, Cottineau J, Picard C, Abel L, Bustamante J, Casanova J, Puel A
    J Exp Med, 2015;212(5):619-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  41. The role of IL-23/Th17 pathway in patients with primary immune thrombocytopenia.
    Authors: Ye X, Zhang L, Wang H, Chen Y, Zhang W, Zhu R, Fang C, Deng A, Qian B
    PLoS ONE, 2015;10(1):e0117704.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  42. Ultraviolet light converts propranolol, a nonselective beta-blocker and potential lupus-inducing drug, into a proinflammatory AhR ligand.
    Authors: Dorgham K, Amoura Z, Parizot C, Arnaud L, Frances C, Pionneau C, Devilliers H, Pinto S, Zoorob R, Miyara M, Larsen M, Yssel H, Gorochov G, Mathian A
    Eur J Immunol, 2015;45(11):3174-87.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  43. Tyrosine Kinase 2-mediated Signal Transduction in T Lymphocytes Is Blocked by Pharmacological Stabilization of Its Pseudokinase Domain.
    Authors: Tokarski J, Zupa-Fernandez A, Tredup J, Pike K, Chang C, Xie D, Cheng L, Pedicord D, Muckelbauer J, Johnson S, Wu S, Edavettal S, Hong Y, Witmer M, Elkin L, Blat Y, Pitts W, Weinstein D, Burke J
    J Biol Chem, 2015;290(17):11061-74.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  44. Vitamin D Antagonises the Suppressive Effect of Inflammatory Cytokines on CTLA-4 Expression and Regulatory Function.
    Authors: Jeffery L, Qureshi O, Gardner D, Hou T, Briggs Z, Soskic B, Baker J, Raza K, Sansom D
    PLoS ONE, 2015;10(7):e0131539.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  45. Th17-related cytokines contribute to recall-like expansion/effector function of HMBPP-specific Vgamma2Vdelta2 T cells after Mycobacterium tuberculosis infection or vaccination.
    Authors: Shen H, Wang Y, Chen C, Frencher J, Huang D, Yang E, Ryan-Payseur B, Chen Z
    Eur J Immunol, 2015;45(2):442-51.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Types: Whole Cells
    Applications: Bioassay
  46. A negative feedback loop mediated by STAT3 limits human Th17 responses.
    Authors: Purvis H, Anderson A, Young D, Isaacs J, Hilkens C
    J Immunol, 2015;193(3):1142-50.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  47. Human group3 innate lymphoid cells express DR3 and respond to TL1A with enhanced IL-22 production and IL-2-dependent proliferation.
    Authors: Ahn Y, Weeres M, Neulen M, Choi J, Kang S, Heo D, Bergerson R, Blazar B, Miller J, Verneris M
    Eur J Immunol, 2015;45(8):2335-42.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  48. EBI3 Downregulation Contributes to Type I Collagen Overexpression in Scleroderma Skin.
    Authors: Kudo H, Wang Z, Jinnin M, Nakayama W, Inoue K, Honda N, Nakashima T, Kajihara I, Makino K, Makino T, Fukushima S, Ihn H
    J Immunol, 2015;195(8):3565-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  49. Cooperation between IL-7 Receptor and Integrin alpha2beta1 (CD49b) Drives Th17-Mediated Bone Loss.
    Authors: El Azreq M, Arseneault C, Boisvert M, Page N, Allaeys I, Poubelle P, Tessier P, Aoudjit F
    J Immunol, 2015;195(9):4198-209.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  50. Differential expression of the immunosuppressive enzyme IL4I1 in human induced Aiolos+, but not natural Helios+, FOXP3+ Treg cells.
    Authors: Scarlata C, Celse C, Pignon P, Ayyoub M, Valmori D
    Eur J Immunol, 2015;45(2):474-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  51. Hypercytotoxicity and rapid loss of NKp44+ innate lymphoid cells during acute SIV infection.
    Authors: Li H, Richert-Spuhler L, Evans T, Gillis J, Connole M, Estes J, Keele B, Klatt N, Reeves R
    PLoS Pathog, 2014;10(12):e1004551.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: Bioassay
  52. Pro-inflammatory human Th17 cells selectively express P-glycoprotein and are refractory to glucocorticoids.
    Authors: Ramesh R, Kozhaya L, McKevitt K, Djuretic I, Carlson T, Quintero M, McCauley J, Abreu M, Unutmaz D, Sundrud M
    J Exp Med, 2014;211(1):89-104.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  53. B7-H1-expressing antigen-presenting cells mediate polarization of protumorigenic Th22 subsets.
    Authors: Kuang D, Xiao X, Zhao Q, Chen M, Li X, Liu R, Wei Y, Ouyang F, Chen D, Wu Y, Lao X, Deng H, Zheng L
    J Clin Invest, 2014;124(10):4657-67.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  54. Decreased RORC-dependent silencing of prostaglandin receptor EP2 induces autoimmune Th17 cells.
    Authors: Kofler D, Marson A, Dominguez-Villar M, Xiao S, Kuchroo V, Hafler D
    J Clin Invest, 2014;124(6):2513-22.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  55. IL-22-producing CD4+ cells are depleted in actively inflamed colitis tissue.
    Authors: Leung J, Davenport M, Wolff M, Wiens K, Abidi W, Poles M, Cho I, Ullman T, Mayer L, Loke P
    Mucosal Immunol, 2014;7(1):124-33.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  56. The E3 deubiquitinase USP17 is a positive regulator of retinoic acid-related orphan nuclear receptor gammat (RORgammat) in Th17 cells.
    Authors: Han L, Yang J, Wang X, Wu Q, Yin S, Li Z, Zhang J, Xing Y, Chen Z, Tsun A, Li D, Piccioni M, Zhang Y, Guo Q, Jiang L, Bao L, Lv L, Li B
    J Biol Chem, 2014;289(37):25546-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  57. Interleukin 7 plays a role in T lymphocyte apoptosis inhibition driven by mesenchymal stem cell without favoring proliferation and cytokines secretion.
    Authors: Normanton M, Alvarenga H, Hamerschlak N, Ribeiro A, Kondo A, Rizzo L, Marti L
    PLoS ONE, 2014;9(9):e106673.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  58. Pathogenic conversion of Foxp3+ T cells into TH17 cells in autoimmune arthritis.
    Authors: Komatsu N, Okamoto K, Sawa S, Nakashima T, Oh-hora M, Kodama T, Tanaka S, Bluestone J, Takayanagi H
    Nat Med, 2014;20(1):62-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  59. The aryl hydrocarbon receptor is functionally upregulated early in the course ofvhuman T-cell activation.
    Authors: Prigent L, Robineau M, Jouneau S, Morzadec C, Louarn L, Vernhet L, Fardel O, Sparfel L
    Eur J Immunol, 2014;44(5):1330-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  60. Plasma cells in the mucosa of patients with inflammatory bowel disease produce granzyme B and possess cytotoxic activities.
    Authors: Cupi M, Sarra M, Marafini I, Monteleone I, Franze E, Ortenzi A, Colantoni A, Sica G, Sileri P, Rosado M, Carsetti R, MacDonald T, Pallone F, Monteleone G
    J Immunol, 2014;192(12):6083-91.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  61. Vitamin D up-regulates the vitamin D receptor by protecting it from proteasomal degradation in human CD4+ T cells.
    Authors: Kongsbak M, von Essen M, Boding L, Levring T, Schjerling P, Lauritsen J, Woetmann A, Odum N, Bonefeld C, Geisler C
    PLoS ONE, 2014;9(5):e96695.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  62. Combinatorial flexibility of cytokine function during human T helper cell differentiation.
    Authors: Touzot M, Grandclaudon M, Cappuccio A, Satoh T, Martinez-Cingolani C, Servant N, Manel N, Soumelis V
    Nat Commun, 2014;5(0):3987.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  63. Differentiation of IL-17-producing effector and regulatory human T cells from lineage-committed naive precursors.
    Authors: Mercer F, Khaitan A, Kozhaya L, Aberg J, Unutmaz D
    J Immunol, 2014;193(3):1047-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  64. Composition of innate lymphoid cell subsets in the human skin: enrichment of NCR(+) ILC3 in lesional skin and blood of psoriasis patients.
    Authors: Teunissen M, Munneke J, Bernink J, Spuls P, Res P, Te Velde A, Cheuk S, Brouwer M, Menting S, Eidsmo L, Spits H, Hazenberg M, Mjosberg J
    J Invest Dermatol, 2014;134(9):2351-60.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  65. Interleukin-12 (IL-12), but not IL-23, induces the expression of IL-7 in microglia and macrophages: implications for multiple sclerosis.
    Authors: Jana M, Mondal S, Jana A, Pahan K
    Immunology, 2014;141(4):549-63.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  66. Halofuginone-induced amino acid starvation regulates Stat3-dependent Th17 effector function and reduces established autoimmune inflammation.
    Authors: Carlson, Thaddeus, Pellerin, Alex, Djuretic, Ivana M, Trivigno, Catherin, Koralov, Sergei B, Rao, Anjana, Sundrud, Mark S
    J Immunol, 2014;192(5):2167-76.
  67. Boswellic acids reduce Th17 differentiation via blockade of IL-1beta-mediated IRAK1 signaling.
    Authors: Sturner K, Verse N, Yousef S, Martin R, Sospedra M
    Eur J Immunol, 2014;44(4):1200-12.
  68. A polysaccharide virulence factor from Aspergillus fumigatus elicits anti-inflammatory effects through induction of Interleukin-1 receptor antagonist.
    Authors: Gresnigt M, Bozza S, Becker K, Joosten L, Abdollahi-Roodsaz S, van der Berg W, Dinarello C, Netea M, Fontaine T, De Luca A, Moretti S, Romani L, Latge J, van de Veerdonk F
    PLoS Pathog, 2014;10(3):e1003936.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  69. Detection of TCD4+ subsets in human carotid atheroma.
    Authors: Oliveira R, Silva R, Teo F, Mineiro M, Ferreira M, Altemani A, Mamoni R, Menezes F, Blotta M
    Cytokine, 2013;62(1):131-40.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Bioassay
  70. RORgammat(+) innate lymphoid cells acquire a proinflammatory program upon engagement of the activating receptor NKp44.
    Authors: Glatzer T, Killig M, Meisig J, Ommert I, Luetke-Eversloh M, Babic M, Paclik D, Bluthgen N, Seidl R, Seifarth C, Grone J, Lenarz M, Stolzel K, Fugmann D, Porgador A, Hauser A, Karlas A, Romagnani C
    Immunity, 2013;38(6):1223-35.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  71. Interleukin-27 is a potent inhibitor of cis HIV-1 replication in monocyte-derived dendritic cells via a type I interferon-independent pathway.
    Authors: Chen, Qian, Swaminathan, Sanjay, Yang, De, Dai, Lue, Sui, Hongyan, Yang, Jun, Hornung, Ronald L, Wang, Yanmei, Huang, Da Wei, Hu, Xiaojun, Lempicki, Richard, Imamichi, Tomozumi
    PLoS ONE, 2013;8(3):e59194.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  72. C3a modulates IL-1beta secretion in human monocytes by regulating ATP efflux and subsequent NLRP3 inflammasome activation.
    Authors: Asgari E, Le Friec G, Yamamoto H, Perucha E, Sacks S, Kohl J, Cook H, Kemper C
    Blood, 2013;122(20):3473-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  73. Age and CD161 expression contribute to inter-individual variation in interleukin-23 response in CD8+ memory human T cells.
    Authors: Shen H, Zhang W, Abraham C, Cho J
    PLoS ONE, 2013;8(3):e57746.
    Species: Human
    Sample Types: Whole Blood
    Applications: Bioassay
  74. Kynurenine 3-monooxygenase mediates inhibition of Th17 differentiation via catabolism of endogenous aryl hydrocarbon receptor ligands.
    Authors: Stephens G, Wang Q, Swerdlow B, Bhat G, Kolbeck R, Fung M
    Eur J Immunol, 2013;43(7):1727-34.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  75. Laminin-411 Is a Vascular Ligand for MCAM and Facilitates TH17 Cell Entry into the CNS.
    Authors: Flanagan K, Fitzgerald K, Baker J
    PLoS ONE, 2012;7(7):e40443.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  76. Complementary IL-23 and IL-27 anti-tumor activities cause strong inhibition of human follicular and diffuse large B-cell lymphoma growth in vivo.
    Authors: Cocco C, Di Carlo E, Zupo S
    Leukemia, 2012;26(6):1365-74.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  77. IL-26 Is Overexpressed in Rheumatoid Arthritis and Induces Proinflammatory Cytokine Production and Th17 Cell Generation.
    Authors: Corvaisier M, Delneste Y, Jeanvoine H, Preisser L, Blanchard S, Garo E, Hoppe E, Barre B, Audran M, Bouvard B, Saint-Andre J, Jeannin P
    PLoS Biol, 2012;10(9):e1001395.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  78. The encephalitogenicity of T(H)17 cells is dependent on IL-1- and IL-23-induced production of the cytokine GM-CSF.
    Authors: El-Behi M, Ciric B, Dai H
    Nat. Immunol., 2011;12(6):568-75.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  79. Th17 cells expressing KIR3DL2+ and responsive to HLA-B27 homodimers are increased in ankylosing spondylitis.
    Authors: Bowness P, Ridley A, Shaw J
    J. Immunol., 2011;186(0):2672.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  80. The IL23R R381Q Gene Variant Protects against Immune-Mediated Diseases by Impairing IL-23-Induced Th17 Effector Response in Humans.
    Authors: Di Meglio P, Di Cesare A, Laggner U
    PLoS ONE, 2011;6(2):e17160.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  81. CD117(+) CD3(-) CD56(-) OX40Lhigh cells express IL-22 and display an LTi phenotype in human secondary lymphoid tissues.
    Authors: Kim S, Han S, Withers DR, Gaspal F, Bae J, Baik S, Shin HC, Kim KS, Bekiaris V, Anderson G, Lane P, Kim MY
    Eur. J. Immunol., 2011;41(6):1563-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  82. B cells as a therapeutic target for IFN-beta in relapsing-remitting multiple sclerosis.
    Authors: Ramgolam VS, Sha Y, Marcus KL, Choudhary N, Troiani L, Chopra M, Markovic-Plese S
    J. Immunol., 2011;186(0):4518.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  83. Regulating human Th17 cells via differential expression of IL-1 receptor.
    Authors: Lee WW, Kang SW, Choi J
    Blood, 2010;115(3):530-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  84. Crucial role of interleukin-7 in T helper type 17 survival and expansion in autoimmune disease.
    Authors: Liu X, Leung S, Wang C, Tan Z, Wang J, Guo TB, Fang L, Zhao Y, Wan B, Qin X, Lu L, Li R, Pan H, Song M, Liu A, Hong J, Lu H, Zhang JZ
    Nat. Med., 2010;16(2):191-7.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  85. IL-10 suppresses Th17 cells and promotes regulatory T cells in the CD4+ T cell population of rheumatoid arthritis patients.
    Authors: Heo YJ, Joo YB, Oh HJ, Park MK, Heo YM, Cho ML, Kwok SK, Ju JH, Park KS, Cho SG, Park SH, Kim HY, Min JK
    Immunol. Lett., 2010;127(2):150-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  86. IL-17 in atopic eczema: linking allergen-specific adaptive and microbial-triggered innate immune response.
    Authors: Eyerich K, Pennino D, Scarponi C, Foerster S, Nasorri F, Behrendt H, Ring J, Traidl-Hoffmann C, Albanesi C, Cavani A
    J. Allergy Clin. Immunol., 2009;123(1):59-66.e4.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  87. Phenotypical characterization of human Th17 cells unambiguously identified by surface IL-17A expression.
    Authors: Brucklacher-Waldert V, Steinbach K, Lioznov M, Kolster M, Holscher C, Tolosa E
    J. Immunol., 2009;183(9):5494-501.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  88. Interleukin-17 and interferon-gamma are produced concomitantly by human coronary artery-infiltrating T cells and act synergistically on vascular smooth muscle cells.
    Authors: Eid RE, Rao DA, Zhou J, Lo SF, Ranjbaran H, Gallo A, Sokol SI, Pfau S, Pober JS, Tellides G
    Circulation, 2009;119(10):1424-32.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  89. Interferon-gamma reverses the immunosuppressive and protumoral properties and prevents the generation of human tumor-associated macrophages.
    Authors: Duluc D, Corvaisier M, Blanchard S, Catala L, Descamps P, Gamelin E, Ponsoda S, Delneste Y, Hebbar M, Jeannin P
    Int. J. Cancer, 2009;125(2):367-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  90. The Th17 cytokine IL-22 induces IL-20 production in keratinocytes: a novel immunological cascade with potential relevance in psoriasis.
    Authors: Wolk K, Witte E, Warszawska K, Schulze-Tanzil G, Witte K, Philipp S, Kunz S, Docke WD, Asadullah K, Volk HD, Sterry W, Sabat R
    Eur. J. Immunol., 2009;39(12):3570-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  91. Blockade of TLR9 agonist-induced type I interferons promotes inflammatory cytokine IFN-gamma and IL-17 secretion by activated human PBMC.
    Authors: Meyers JA, Mangini AJ, Nagai T, Roff CF, Sehy D, van Seventer JM
    Cytokine, 2006;35(5):235-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  92. A novel form of complete IL-12/IL-23 receptor beta1 deficiency with cell surface-expressed nonfunctional receptors.
    Authors: Fieschi C, Bosticardo M, de Beaucoudrey L, Boisson-Dupuis S, Feinberg J, Santos OF, Bustamante J, Levy J, Candotti F, Casanova JL
    Blood, 2004;104(7):2095-101.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  93. TGF-beta Affects the Differentiation of Human GM-CSF+ CD4+ T Cells in an Activation- and Sodium-Dependent Manner.
    Authors: Elias S, Schmidt A, Kannan V, Andersson J, Tegner J
    Front Immunol, 0;7(0):603.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

  1. Does Recombinant Human IL-23 Protein (Catalog # 1290-IL or Catalog # 1290-IL/CF ) have any tags?

    • Recombinant Human IL-23 Protein (Catalog # 1290-IL or 1290-IL/CF) does not have any tags.  Please refer to the Source section on the product-specific page for sequence information.

  2. Has Recombinant Human IL-23 Protein (Catalog # 1290-IL or Catalog # 1290-IL/CF) been tested for activity on mouse or non-human primate species?

    • Recombinant Human IL-23 Protein (Catalog # 1290-IL or 1290-IL/CF) activity is measured by its ability to induce IL-17 secretion by mouse splenocytes. The ED50 for this effect is 0.05-0.3 ng/mL.  We have not tested activity of the protein on non-human primate cells in our lab.  Please refer the Citations tab to find publications reporting the use of the protein on other species.  Filter the results based on species and sample type for a more relevant search to your target species.

  3. Human IL-23 and Human IL-12 share a p40 subunit.  Why is the predicted molecular weight for the p40 subunit 36.5 kDa in the Recombinant Human IL-23 Protein (Catalog # 1290-IL), while the predicted molecular weight for the p40 subunit is 34.7 kDa for the Recombinant Human IL-12 Protein  (Catalog # 219-IL)?

    • The Recombinant Human IL-23 Protein (Catalog # 1290-IL) and Recombinant Human IL-12 Protein (Catalog # 219-IL) p40 subunits share the same p40 sequence, Ile23-Ser328 (Accession # P29460).  However, the p40 subunit for Recombinant Human IL-23 (Catalog # 1290-IL) also contains a portion of the GS linker that remains after cleaving p40 subunit from the p19 subunit, so its predicted molecular is slightly larger (36.5 kDa) compared to that of the Recombinant Human IL-12 Protein (Catalog # 219-IL; 34.7 kDa).  Please see the product-specific page for detailed sequence information.

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Average Rating: 4.5 (Based on 6 Reviews)

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Recombinant Human IL-23 Protein
By Anonymous on 01/07/2020
Application: In vitro bioactivity in cell culture
Reason for Rating: high unit concentration, easy to use

Recombinant Human IL-23 Protein
By Anonymous on 03/19/2019
Application: Binding assay/Protein-protein interaction

Recombinant Human IL-23 Protein
By Anonymous on 12/04/2018
Application: CellProlif

Recombinant Human IL-23 Protein
By Anonymous on 12/01/2017
Application: Stem/Immune cell maintenance or differentiation

Recombinant Human IL-23 Protein
By Anonymous on 12/15/2016
Application: Stem/Immune cell maintenance or differentiation

We add to our cell culture as a supplement.


Recombinant Human IL-23 Protein
By Anonymous on 08/14/2016
Application: Other

Human Th17 cells were differentiated and maintained.