Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1-5). The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. Human p19 cDNA encodes a 189 amino acid residue (aa) precursor protein with a putative 19 aa signal peptide and 170 aa mature protein. Human and mouse p19 share 70% aa sequence identity. Although p19 is expressed by activated macrophages, dendritic cells, T cells, and endothelial cells, only activated macrophages and dendritic cells express p40 concurrently to produce IL-23. The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R). IL-23 has biological activities that are similar to, but distinct from IL-12. Both IL-12 and IL-23 induce proliferation and IFN-gamma production by human T cells. While IL-12 acts on both naïve and memory human T cells, the effects of IL-23 is restricted to memory T cells. In mouse, IL-23 but not IL-12, has also been shown to induce memory T cells to secret IL-17, a potent proinflammatory cytokine. IL-12 and IL-23 can induce IL-12 production from mouse splenic DC of both the CD8- and CD8+ subtypes, however only IL-23 can act directly on CD8+ DC to mediate immunogenic presentation of poorly immunogenic tumor/self peptide.
Recombinant Human IL-23 Protein
R&D Systems | Catalog # 1290-IL
Key Product Details
- R&D Systems Sf 21 (baculovirus)-derived Recombinant Human IL-23 Protein (1290-IL)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
Accession Number
Applications
Product Specifications
Source
| Human IL-23 p40 (Ile23-Ser328) Accession # P29460 |
GSGSSRGGSGSGGSGGGGSKL | Human IL-23 p19 (Arg20-Pro189) Accession # AAG37232 |
| N-terminus | C-terminus |
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
The ED50 for this effect is 0.05-0.3 ng/mL.
Reviewed Applications
Read 6 reviews rated 4.5 using 1290-IL in the following applications:
Scientific Data Images for Recombinant Human IL-23 Protein
Recombinant Human IL‑23 Protein SEC-MALS.
Recombinant Human IL-23 Protein (Catalog # 1290-IL) has a molecular weight (MW) of 57.9 kDa as analyzed by SEC-MALS, suggesting that this protein is a heterodimer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).Formulation, Preparation, and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 1290-IL
| Formulation | Lyophilized from a 0.2 μm filtered solution in MES, NaCl and EDTA with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Carrier Free: 1290-IL/CF
| Formulation | Supplied as a 0.2 μm filtered solution in MES, NaCl and EDTA. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Calculators
Background: IL-23
References
- Oppmann, B. et al. (2000) Immunity 13:715.
- Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.
- Parham, C. et al. (2002) J. Immunol. 168:5699.
- Belladonna, M.L. et al. (2002) J. Immunol. 168:5448.
- Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.
Long Name
Alternate Names
Gene Symbol
UniProt
Additional IL-23 Products
Product Documents for Recombinant Human IL-23 Protein
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human IL-23 Protein
For research use only
Citations for Recombinant Human IL-23 Protein
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Customer Reviews for Recombinant Human IL-23 Protein (6)
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Customer Images
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 01/07/2020
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Application: Binding assay/Protein-protein interactionVerified Customer | Posted 03/19/2019
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Application: Cell ProliferationVerified Customer | Posted 12/04/2018
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Application: Stem/Immune cell maintenance or differentiationVerified Customer | Posted 12/01/2017
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Application: Stem/Immune cell maintenance or differentiationVerified Customer | Posted 12/15/2016We add to our cell culture as a supplement.
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Verified Customer | Posted 08/14/2016Human Th17 cells were differentiated and maintained.
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FAQs for Recombinant Human IL-23 Protein
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Q: Does Recombinant Human IL-23 Protein (Catalog # 1290-IL or Catalog # 1290-IL/CF ) have any tags?
A: Recombinant Human IL-23 Protein (Catalog # 1290-IL or 1290-IL/CF) does not have any tags. Please refer to the Source section on the product-specific page for sequence information.
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Q: Has Recombinant Human IL-23 Protein (Catalog # 1290-IL or Catalog # 1290-IL/CF) been tested for activity on mouse or non-human primate species?
A: Recombinant Human IL-23 Protein (Catalog # 1290-IL or 1290-IL/CF) activity is measured by its ability to induce IL-17 secretion by mouse splenocytes. The ED50 for this effect is 0.05-0.3 ng/mL. We have not tested activity of the protein on non-human primate cells in our lab. Please refer the Citations tab to find publications reporting the use of the protein on other species. Filter the results based on species and sample type for a more relevant search to your target species.
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Q: Human IL-23 and Human IL-12 share a p40 subunit. Why is the predicted molecular weight for the p40 subunit 36.5 kDa in the Recombinant Human IL-23 Protein (Catalog # 1290-IL), while the predicted molecular weight for the p40 subunit is 34.7 kDa for the Recombinant Human IL-12 Protein (Catalog # 219-IL)?
A: The Recombinant Human IL-23 Protein (Catalog # 1290-IL) and Recombinant Human IL-12 Protein (Catalog # 219-IL) p40 subunits share the same p40 sequence, Ile23-Ser328 (Accession # P29460). However, the p40 subunit for Recombinant Human IL-23 (Catalog # 1290-IL) also contains a portion of the GS linker that remains after cleaving p40 subunit from the p19 subunit, so its predicted molecular is slightly larger (36.5 kDa) compared to that of the Recombinant Human IL-12 Protein (Catalog # 219-IL; 34.7 kDa). Please see the product-specific page for detailed sequence information.
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Q: Does Recombinant Human IL-23 Protein (Catalog # 1290-IL or Catalog # 1290-IL/CF ) have any tags?
A: Recombinant Human IL-23 Protein (Catalog # 1290-IL or 1290-IL/CF) does not have any tags. Please refer to the Source section on the product-specific page for sequence information.
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Q: Has Recombinant Human IL-23 Protein (Catalog # 1290-IL or Catalog # 1290-IL/CF) been tested for activity on mouse or non-human primate species?
A: Recombinant Human IL-23 Protein (Catalog # 1290-IL or 1290-IL/CF) activity is measured by its ability to induce IL-17 secretion by mouse splenocytes. The ED50 for this effect is 0.05-0.3 ng/mL. We have not tested activity of the protein on non-human primate cells in our lab. Please refer the Citations tab to find publications reporting the use of the protein on other species. Filter the results based on species and sample type for a more relevant search to your target species.
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Q: Human IL-23 and Human IL-12 share a p40 subunit. Why is the predicted molecular weight for the p40 subunit 36.5 kDa in the Recombinant Human IL-23 Protein (Catalog # 1290-IL), while the predicted molecular weight for the p40 subunit is 34.7 kDa for the Recombinant Human IL-12 Protein (Catalog # 219-IL)?
A: The Recombinant Human IL-23 Protein (Catalog # 1290-IL) and Recombinant Human IL-12 Protein (Catalog # 219-IL) p40 subunits share the same p40 sequence, Ile23-Ser328 (Accession # P29460). However, the p40 subunit for Recombinant Human IL-23 (Catalog # 1290-IL) also contains a portion of the GS linker that remains after cleaving p40 subunit from the p19 subunit, so its predicted molecular is slightly larger (36.5 kDa) compared to that of the Recombinant Human IL-12 Protein (Catalog # 219-IL; 34.7 kDa). Please see the product-specific page for detailed sequence information.
-
Q: Does Recombinant Human IL-23 Protein (Catalog # 1290-IL or Catalog # 1290-IL/CF ) have any tags?
A: Recombinant Human IL-23 Protein (Catalog # 1290-IL or 1290-IL/CF) does not have any tags. Please refer to the Source section on the product-specific page for sequence information.
-
Q: Has Recombinant Human IL-23 Protein (Catalog # 1290-IL or Catalog # 1290-IL/CF) been tested for activity on mouse or non-human primate species?
A: Recombinant Human IL-23 Protein (Catalog # 1290-IL or 1290-IL/CF) activity is measured by its ability to induce IL-17 secretion by mouse splenocytes. The ED50 for this effect is 0.05-0.3 ng/mL. We have not tested activity of the protein on non-human primate cells in our lab. Please refer the Citations tab to find publications reporting the use of the protein on other species. Filter the results based on species and sample type for a more relevant search to your target species.
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Q: Human IL-23 and Human IL-12 share a p40 subunit. Why is the predicted molecular weight for the p40 subunit 36.5 kDa in the Recombinant Human IL-23 Protein (Catalog # 1290-IL), while the predicted molecular weight for the p40 subunit is 34.7 kDa for the Recombinant Human IL-12 Protein (Catalog # 219-IL)?
A: The Recombinant Human IL-23 Protein (Catalog # 1290-IL) and Recombinant Human IL-12 Protein (Catalog # 219-IL) p40 subunits share the same p40 sequence, Ile23-Ser328 (Accession # P29460). However, the p40 subunit for Recombinant Human IL-23 (Catalog # 1290-IL) also contains a portion of the GS linker that remains after cleaving p40 subunit from the p19 subunit, so its predicted molecular is slightly larger (36.5 kDa) compared to that of the Recombinant Human IL-12 Protein (Catalog # 219-IL; 34.7 kDa). Please see the product-specific page for detailed sequence information.
