Human Ryk Alexa Fluor® 594-conjugated Antibody

Catalog #: AF4907T Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4907T-100UG

Save 15% on Select RUO Reagents. See Details.

All Ryk Antibodies

Product Details

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Ryk Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Ryk in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross-reactivity with recombinant mouse Ryk is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Ryk
Ala47-Thr224
Accession # NP_001005861

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

CyTOF-ready

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Ryk

RYK (related to tyrosine (Y) kinase; also H-RYK) is a 70-90 kDa, atypical member of the tyrosine protein kinase family. It is a type I transmembrane glycoprotein that binds Wnt, and forms a Wnt receptor complex with frizzled. In this capacity, it serves as a link between Wnt and Dishevelled. Human RYK is 604 amino acids (aa) in length (SwissProt Accession # P34925). It contains a 25 aa signal sequence and a 199 aa extracellular region (aa 26-224) that shows one WIF-1-like domain (aa 63‑191). The cytoplasmic region possesses a nonfunctional Ser/Thr protein kinase domain (aa 327-600). One isoform exists that shows a 31 aa substitution between aa’s 18-46. Over aa 47-224, human RYK is 94% aa identical to mouse RYK.

Long Name

Receptor Type Tyrosine Protein Kinase RYK

Entrez Gene IDs

6259 (Human); 20187 (Mouse); 140585 (Rat)

Alternate Names

D3S3195; EC 2.7.10; EC 2.7.10.1; hydroxyaryl-protein kinase; JTK5; JTK5A protein tyrosine kinase; JTK5A; RYK receptor-like tyrosine kinase; Ryk; RYK1; tyrosine-protein kinase RYK