Human Ryk Biotinylated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Ryk in Western blots. In Western blots, approximately 40% cross-reactivity with recombinant mouse Ryk is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Ryk
    Ala47-Thr224
    Accession # NP_002949
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human Ryk
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Ryk

Ryk (Related to tyrosine (Y) kinase; also VIK and MRK) is a member of the tyrosine protein kinase family. It is a type I transmembrane glycoprotein that binds Wnt and forms a Wnt receptor complex with Frizzled. In this capacity, Ryk serves as a link between Wnt and Dishevelled.

Human Ryk is 604 amino acids (aa) in length. It contains a 25 aa signal sequence and a 199 aa extracellular region (aa 26 - 224) that shows one WIF-1-like domain (aa 63 - 191). The cytoplasmic region of Ryk possesses a nonfunctional Ser/Thr protein kinase domain (aa 327 - 600). One isoform exists that shows a 31 aa substitution between aa 18 - 46. Over aa 47 - 224, human Ryk is 94% aa identical to mouse Ryk.

  • Long Name:
    Receptor Type Tyrosine Protein Kinase RYK
  • Entrez Gene IDs:
    6259 (Human); 20187 (Mouse); 140585 (Rat)
  • Alternate Names:
    D3S3195; EC 2.7.10; EC 2.7.10.1; hydroxyaryl-protein kinase; JTK5; JTK5A protein tyrosine kinase; JTK5A; RYK receptor-like tyrosine kinase; Ryk; RYK1; tyrosine-protein kinase RYK
Related Research Areas
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