S100A2 (also S100L and CaN19) is a 10 kDa member of the S100 family, EF-hand superfamily of Ca-binding proteins. It is expressed by multiple cell types including keratinocytes, chondrocytes, and bronchial epithelium. S100A2 regulates both Ca and Zn (in human) within cells and increases p53 activity. It forms both noncovalent and covalent homodimers and a homotetramer under certain conditions. Human S100A2 is 98 amino acids (aa) in length. It contains two EF-hand motifs (aa 13‑48 and 51‑86) plus a calcium (aa 64‑75) and zinc (aa 17‑22) binding site. There is one potential alternative splice form that shows a 40 aa insertion after Gly48. Full-length human S100A2 shares 89% and 85% aa identity with bovine and canine S100A2, respectively.
Human S100A2 Alexa Fluor™ Plus 488‑conjugated Antibody
R&D Systems | Catalog # AF4870AFP488
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunocytochemistry
Label
Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Specificity
Detects human S100A2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) S100A1, -A6, -A7, -A8, -A10, -A11, -A13, -A16, rhS100B and rhS100P is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human S100A2 Alexa Fluor™ Plus 488‑conjugated Antibody
Application
Recommended Usage
Immunocytochemistry
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: S100A2
Long Name
S100 Calcium Binding Protein A2
Alternate Names
CAN19, S100L
Entrez Gene IDs
6273 (Human)
Gene Symbol
S100A2
UniProt
Additional S100A2 Products
Product Documents for Human S100A2 Alexa Fluor™ Plus 488‑conjugated Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human S100A2 Alexa Fluor™ Plus 488‑conjugated Antibody
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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