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Human S100A6 Alexa Fluor® 405-conjugated Antibody

Catalog #: FAB7467V Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB7467V-100UG

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Human S100A6 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human S100A6 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse S100A6 and no cross-reactivity with recombinant human (rh) S100A1, rhS100A4, or rhS100B is observed.
Source
Monoclonal Mouse IgG2a Clone # 754102
Purification
Protein A or G purified
Immunogen
E. coli-derived recombinant human S100A6
Met1-Gly90
Accession # P06703
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: S100A6

S100A6, also called PRA (prolactin receptor-associated protein) and calcyclin, is a 10 kDa member of the S100 family of calcium-binding proteins. Human S100A6 is 90 amino acids (aa) in length and contains two calcium-binding EF-hand domains (aa 12 - 47 and 48 - 83). Intracellularly, S100A6 will both noncovalently homodimerize and heterodimerize with S100B plus SGT1. Extracellularly, it is secreted via a noncanonical pathway and binds to RAGE, inducing apoptosis. It is expressed by neurons, endothelium, fibroblasts and glandular epithelia. Human S100A6 shares 95% aa sequence identity with mouse and rat S100A6. This antibody detects mouse S100A6 in Western Blots. Mouse cross-reactivity has not been tested in Immunohistochemistry

Long Name
S100 Calcium Binding Protein A6
Entrez Gene IDs
6277 (Human); 20200 (Mouse); 85247 (Rat)
Alternate Names
2A9; CABP; CACY; CACY5B10; calcyclin; Growth factor-inducible protein 2A9; MLN 4; PRA; PRAS100 calcium binding protein A6 (calcyclin); Prolactin receptor-associated protein; protein S100-A6; S100 calcium binding protein A6; S100 calcium-binding protein A6 (calcyclin); S100 calcium-binding protein A6; S100A6

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