Human S1P1/EDG-1 Alexa Fluor® 488-conjugated Antibody

Clone 218713 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
FAB2016G-100UG
Detection  of S1P1/EDG‑1 in CHO Chinese Hamster Cell  Line Transfected with Human S1P1/EDG-1 by Flow Cytometry.
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Human S1P1/EDG-1 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human S1P1/EDG‑1 by flow cytometry.
Source
Monoclonal Mouse IgG2B Clone # 218713
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human S1P1/EDG‑1
Met1-Ser382
Accession # P21453
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection  of S1P1/EDG‑1 in CHO Chinese Hamster Cell  Line Transfected with Human S1P1/EDG-1 by Flow Cytometry. View Larger

Detection of S1P1/EDG‑1 in CHO Chinese Hamster Cell Line Transfected with Human S1P1/EDG-1 by Flow Cytometry. CHO Chinese hamster ovary cell line transfected with human S1P1/EDG-1 were stained with Mouse Anti-Human S1P1/EDG‑1 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB2016G, filled histogram) or Mouse IgG2B Alexa Fluor® 488‑conjugated Isotype Control Antibody (Catalog # IC0041G, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: S1P1/EDG-1

S1P1, also known as EDG-1, is a member of the endothelial differentiation gene family. It is a high affinity G protein-coupled receptor for the bioactive lipid, Sphingosine-1-phosphate. S1P1 signaling regulates endothelial cell survival, cytoskeletal remodeling, chemotaxis, and angiogenesis.

Long Name
Endothelial Differentiation Gene 1
Entrez Gene IDs
1901 (Human); 13609 (Mouse)
Alternate Names
CD363 antigen; CD363; CHEDG1; D1S3362; EDG1; EDG-1; EDG1edg-1; Endothelial differentiation G-protein coupled receptor 1; endothelial differentiation, sphingolipid G-protein-coupled receptor, 1; FLJ58121; S1P receptor 1; S1P receptor Edg-1; S1P1; S1P1ECGF1; S1PR1; sphingosine 1-phosphate receptor 1; sphingosine 1-phosphate receptor EDG1; Sphingosine 1-phosphate receptor Edg-1; sphingosine-1-phosphate receptor 1

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