Human sE-Selectin/CD62E Quantikine ELISA Kit DSLE00: R&D Systems

Human sE-Selectin/CD62E Quantikine ELISA Kit

(22 citations)
(1 Review)
  
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (10 uL), Heparin Plasma (10 uL), Citrate Plasma (10 uL)
  • Sensitivity
    0.027 ng/mL
  • Assay Range
    0.1 - 8 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma, Citrate Plasma)
  • Specificity
    Natural and recombinant human sE-Selectin
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC236, Quantikine Immunoassay Control Group 236 - Please Inquire
Product Summary
The Quantikine Human sE-Selectin immunoassay is a 4.5 hour solid phase ELISA designed to measure sE-Selectin in cell culture supernates, serum, and plasma. It contains CHO cell-expressed recombinant human sE-Selectin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human sE-Selectin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human sE-Selectin.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, Heparin Plasma, Citrate Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean1.62.94.71.52.94.6
Standard Deviation0.110.170.240.110.250.35
CV%6.65.75.27.38.77.7

Recovery

The recovery of sE-Selectin spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 103 98-110
Citrate Plasma (n=4) 96 85-107
Heparin Plasma (n=4) 94 85-107
Serum (n=4) 96 85-106
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of sE-Selectin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human sE-Selectin/CD62E Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: E-Selectin/CD62E
The Selectin family is comprised of three members, E-Selectin, L-Selectin, and P-Selectin. E-Selectin [endothelial leukocyte adhesion molecule-1 (ELAM-1), CD62E] is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha. The human and rat proteins share approximately 67% amino acid sequence identity. The mouse and rat proteins share approximately 78% amino acid sequence identity. L-Selectin (Leukocyte Selectin, LAM-1, LECAM-1, LECCAM-1, TQ1, Leu-8, MEL-14 antigen, DREG, lymph node homing receptor, CD62L) is expressed constitutively on a wide variety of leukocytes. Two forms of L-Selectin have been reported, apparently arising as a result of post-translational modifications. Human and mouse L-Selectin share 76% amino acid sequence identity. Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P) is expressed by activated platelets and endothelial cells. The extracellular domains of human and mouse P-Selectin share approximately 73% amino acid sequence identity.
    • Entrez Gene IDs
      6401 (Human); 20339 (Mouse); 25544 (Rat);
    • Alternate Names
      CD62 antigen-like family member E; CD62E antigen; CD62E; ELAM; ELAM1; ELAM-1; ELAM1E-selectin; endothelial adhesion molecule 1; Endothelial leukocyte adhesion molecule 1; ESEL; E-Selectin; LECAM2; leukocyte endothelial cell adhesion molecule 2; Leukocyte-endothelial cell adhesion molecule 2; SELE; selectin E;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 100 µL Standard, Control, or Sample
    6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    22 Citations: Showing 1 - 10
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    Species
    Sample Type
    1. Vitamin D status and circulating biomarkers of endothelial dysfunction and inflammation in non-diabetic obese individuals: a pilot study
      Authors: B Ilin?i?, E Stoki?, Z Sto?i?, NE Koji?, N Katsiki, DP Mikhailidi, ER Isenovic
      Arch Med Sci, 2017;13(1):53-60.
      Species: Human
      Sample Type: Serum
    2. A Pilot Study to Evaluate Haemostatic Function, following Shock Wave Lithotripsy (SWL) for the Treatment of Solitary Kidney Stones.
      Authors: Hughes S, Thomas-Wright S, Banwell J, Williams R, Moyes A, Mushtaq S, Abdulmajed M, Shergill I
      PLoS ONE, 2015;10(5):e0125840.
      Species: Human
      Sample Type: Plasma
    3. Kidney function, endothelial activation and atherosclerosis in black and white Africans with rheumatoid arthritis.
      Authors: Dessein, Patrick, Hsu, Hon-Chun, Tsang, Linda, Millen, Aletta M, Woodiwiss, Angela J, Norton, Gavin R, Solomon, Ahmed, Gonzalez-Gay, Miguel A
      PLoS ONE, 2015;10(3):e0121693.
      Species: Human
      Sample Type: Plasma
    4. Immune-inflammatory markers and arterial stiffness indexes in subjects with acute ischemic stroke with and without metabolic syndrome.
      Authors: Tuttolomondo A, Pecoraro R, Di Raimondo D, Di Sciacca R, Canino B, Arnao V, Butta C, Della Corte V, Maida C, Licata G, Pinto A
      Diabetol Metab Syndr, 2014;6(1):28.
      Species: Human
      Sample Type: Plasma
    5. Plasma concentrations predict aortic expression of growth-arrest-specific protein 6 in patients undergoing coronary artery bypass grafting.
      Authors: Lee, Chien-Hs, Shieh, Yi-Shing, Tsai, Chien-Su, Hung, Yi-Jen, Tsai, Yi-Ting, Lin, Chih-Yua
      PLoS ONE, 2013;8(11):e79452.
      Species: Human
      Sample Type: Plasma
    6. Association between traffic-related air pollution, subclinical inflammation and impaired glucose metabolism: results from the SALIA study.
      Authors: Teichert T, Vossoughi M, Vierkotter A, Sugiri D, Schikowski T, Schulte T, Roden M, Luckhaus C, Herder C, Kramer U
      PLoS ONE, 2013;8(12):e83042.
      Species: Human
      Sample Type: Plasma
    7. Systemic biomarkers of inflammation and haemostasis in patients with chronic necrotizing pulmonary aspergillosis.
      BMC Infect Dis, 2012;12(0):144.
      Species: Human
      Sample Type: Serum
    8. Endogenous granulocyte colony-stimulating factor: a biomarker in acute ischemic stroke.
      Authors: Yu SC, Kuo CL, Huang CS, Chang CS, Wu SL, Su SL, Liu CS
      Biomarkers, 2012;17(4):319-24.
      Species: Human
      Sample Type: Plasma
    9. Adipocytokine levels mark endothelial function in normotensive individuals.
      Authors: Solini A, Stea F, Santini E, Bruno R, Duranti E, Taddei S, Ghiadoni L
      Cardiovasc Diabetol, 2012;11(0):103.
      Species: Human
      Sample Type: Plasma
    10. Changes in cytokine levels during acute hyperinsulinemia in offspring of type 2 diabetic subjects.
      Authors: Ruotsalainen E, Stancakova A, Vauhkonen I, Salmenniemi U, Pihlajamaki J, Punnonen K, Laakso M
      Atherosclerosis, 2010;210(2):536-41.
      Species: Human
      Sample Type: Plasma
    11. First-trimester prediction of gestational diabetes mellitus: examining the potential of combining maternal characteristics and laboratory measures.
      Authors: Savvidou M, Nelson SM, Makgoba M, Messow CM, Sattar N, Nicolaides K
      Diabetes, 2010;59(12):3017-22.
      Species: Human
      Sample Type: Serum
    12. Matrix metalloproteinase-9 and plasminogen activator inhibitor-1 are associated with right ventricular structure and function: the MESA-RV Study.
      Authors: Kawut SM, Barr RG, Johnson WC
      Biomarkers, 2010;15(8):731-8.
      Species: Human
      Sample Type: Plasma
    13. SIRT1 mRNA expression may be associated with energy expenditure and insulin sensitivity.
      Authors: Rutanen J, Yaluri N, Modi S, Pihlajamaki J, Vanttinen M, Itkonen P, Kainulainen S, Yamamoto H, Lagouge M, Sinclair DA, Elliott P, Westphal C, Auwerx J, Laakso M
      Diabetes, 2010;59(4):829-35.
      Species: Human
      Sample Type: Serum
    14. Prehospital resuscitation with hypertonic saline-dextran modulates inflammatory, coagulation and endothelial activation marker profiles in severe traumatic brain injured patients.
      Authors: Rhind SG, Crnko NT, Baker AJ
      J Neuroinflammation, 2010;7(0):5.
      Species: Human
      Sample Type: Serum
    15. Effect of intensive lifestyle changes on endothelial function and on inflammatory markers of atherosclerosis.
      Authors: Dod HS, Bhardwaj R, Sajja V, Weidner G, Hobbs GR, Konat GW, Manivannan S, Gharib W, Warden BE, Nanda NC, Beto RJ, Ornish D, Jain AC
      Am. J. Cardiol., 2010;105(3):362-7.
      Species: Human
      Sample Type: Serum
    16. Immuno-inflammatory predictors of stroke at follow-up in patients with chronic non-valvular atrial fibrillation (NVAF).
      Authors: Pinto A, Tuttolomondo A, Casuccio A, Di Raimondo D, Di Sciacca R, Arnao V, Licata G
      Clin. Sci., 2009;116(10):781-9.
      Species: Human
      Sample Type: Plasma
    17. Immuno-inflammatory and thrombotic/fibrinolytic variables associated with acute ischemic stroke diagnosis.
      Authors: Tuttolomondo A, Pinto A, Corrao S, Di Raimondo D, Fernandez P, Di Sciacca R, Arnao V, Licata G
      Atherosclerosis, 2009;203(2):503-8.
      Species: Human
      Sample Type: Plasma
    18. Non-conventional markers of atherosclerosis before and after gastric banding surgery.
      Authors: Hanusch-Enserer U, Zorn G, Wojta J, Kopp CW, Prager R, Koenig W, Schillinger M, Roden M, Huber K
      Eur. Heart J., 2009;30(12):1516-24.
      Species: Human
      Sample Type: Plasma
    19. Consumption of isoflavone-rich soy protein does not alter homocysteine or markers of inflammation in postmenopausal women.
      Authors: Greany KA, Nettleton JA, Wangen KE, Thomas W, Kurzer MS
      Eur J Clin Nutr, 2008;62(0):1419-25.
      Species: Human
      Sample Type: Serum
    20. Angiopoietin-2 is associated with decreased endothelial nitric oxide and poor clinical outcome in severe falciparum malaria.
      Authors: Yeo TW, Lampah DA, Gitawati R, Tjitra E, Kenangalem E, Piera K, Price RN, Duffull SB, Celermajer DS, Anstey NM
      Proc. Natl. Acad. Sci. U.S.A., 2008;105(44):17097-102.
      Species: Human
      Sample Type: Plasma
    21. Rosiglitazone produces a greater reduction in circulating platelet activity compared with gliclazide in patients with type 2 diabetes mellitus--an effect probably mediated by direct platelet PPARgamma activation.
      Authors: Khanolkar MP, Morris RH, Thomas AW, Bolusani H, Roberts AW, Geen J, Jackson SK, Evans LM
      Atherosclerosis, 2007;197(2):718-24.
      Species: Human
      Sample Type: Serum
    22. Angiopoietin-1 and angiopoietin-2 as clinically informative prognostic biomarkers of morbidity and mortality in severe sepsis*
      Authors: Ricciuto DR, Dos Santos CC, Hawkes M, Toltl LJ, Conroy AL, Rajwans N, Lafferty EI, Cook DJ, Fox-Robichaud A, Kahnamoui K, Kain KC, Liaw PC, Liles WC
      Crit. Care Med., 0;0(0):.
      Species: Human
      Sample Type: Plasma
    Expand to show all 22 Citations
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    Quantikine Immunoassay Control Group 236

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