BSCL2 (Bernardinelli-Seip Congenital Lipodystrophy type 2 protein; also Seipin) is a 70-74 kDa glycoprotein member of the seipin family of molecules. Although it has widespread expression, and has been specifically found in the endoplasmic reticulum (ER) of cortical and spinal motor neurons, basophils of the anterior pituitary, adipocytes and spermatids. Its function is unclear, but it is known to structurally resemble SREBPs, and thus may regulate gene transcription. Mutations are reported that involve its glycosylation site, causing protein misfolding and the initiation of apoptosis. Human BSCL2 is 398 amino acids (aa) in length. It contains two transmembrane segments (aa 27-47 and 243-263) plus an N-terminal (aa 1-26) and C-terminal (aa 264-398) cytoplasmic domain. BSCL2 is polyubiquitinated, and runs between 100-200 kDa in SDS-PAGE. There is an alternative start site 64 aa upstream of the standard site. This 462 aa isoform is considered to be the predominant isoform for BSCL2, and is associated with the 70-74 kDa MW cited above. There is one additional potential isoform variant that possesses a 63 aa substitution for aa 225-398. BSCL2 is reportedly cleaved, and generates a 40-41 kDa N-terminal fragment. Over aa 279-398, human BSCL2 shares 73% aa sequence identity with mouse BSCL2.
Human Seipin/BSCL2 Alexa Fluor® 750‑conjugated Antibody
R&D Systems | Catalog # AF7385S
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Asn279-Ser398
Accession # Q96G97
Specificity
Clonality
Host
Isotype
Applications for Human Seipin/BSCL2 Alexa Fluor® 750‑conjugated Antibody
Immunocytochemistry
Western Blot
Formulation, Preparation, and Storage
Purification
Formulation
Shipping
Stability & Storage
Background: Seipin/BSCL2
Long Name
Alternate Names
Gene Symbol
UniProt
Additional Seipin/BSCL2 Products
Product Documents for Human Seipin/BSCL2 Alexa Fluor® 750‑conjugated Antibody
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Product Specific Notices for Human Seipin/BSCL2 Alexa Fluor® 750‑conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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Protocols
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- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars