Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all Semaphorin 4B Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Semaphorin 4B isoform 1
Leu39-Glu712
Accession # Q9NPR2

Specificity

Detects human Semaphorin 4B in Western blots. In Western blots, approximately 20% cross‑reactivity with recombinant mouse Semaphorin 4B is observed and less than 1% cross‑reactivity with recombinant human Semaphorin 3A, 4A, and 7A is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Semaphorin 4B Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Semaphorin 4B

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Semaphorin 4B

Semaphorin 4B (Sema4B; also SemaC) is a 95‑100 kDa, class IV member of the Semaphorin family of proteins. It is expressed in neurons, and following PSD-95 induced clustering, participates in the formation or functioning of glutamatergic synapses. Mature human Sema4B is a type I transmembrane glycoprotein that is 794 amino acids (aa) in length. It contains a 674 aa extracellular region (aa 39‑832) that is characterized by one Sema domain (aa 65‑502), a PSI region (aa 520‑574), and an Ig-like C2-type domain (aa 599‑658). There is one potential soluble splice variant that shows a 29 aa substitution for aa 707‑832. Over aa 39‑712, human Sema4B shares 86% aa identity with mouse Sema4B.

Alternate Names

SEMA4B, SEMAC, SemC

Entrez Gene IDs

10509 (Human); 20352 (Mouse); 293042 (Rat)

Gene Symbol

SEMA4B

UniProt

Q9NPR2

Additional Semaphorin 4B Products

Product Documents for Human Semaphorin 4B Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Semaphorin 4B Biotinylated Antibody

For research use only

Related Research Areas

Proteoglycans

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