Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Semaphorin 6D
Ser22-His660
Accession # Q8NFY4

Specificity

Detects human Semaphorin 6D in Western blots. In Western blots, approximately 15% cross‑reactivity with recombinant human (rh) Semaphorin 6C is observed and less than 2% cross-reactivity with rhSemaphorin 6A, rhSemaphorin 6B, rhSemaphorin 3A, rhSemaphorin 3B, rhSemaphorin 7A, recombinant mouse (rm) Semaphorin 3C, rmSemaphorin 3E, and rmSemaphorin 3F is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Semaphorin 6D Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Semaphorin 6D

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.


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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Semaphorin 6D

Semaphorin 6D is a type I transmembrane protein belonging to the semaphorin family. Members of the sema family were originally identified as axon guidance molecules. Semaphorin 6D is expressed during development and in adult kidney, brain, placenta, heart and skeletal muscles. Five splice variants of Semaphorin 6D exist.

Alternate Names

Sema6D

Entrez Gene IDs

80031 (Human); 214968 (Mouse); 311384 (Rat)

Gene Symbol

SEMA6D

UniProt

Additional Semaphorin 6D Products

Product Documents for Human Semaphorin 6D Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Semaphorin 6D Biotinylated Antibody

For research use only

Related Research Areas

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