Human Semaphorin 7A APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human Semaphorin 7A in direct ELISAs.
  • Source
    Monoclonal Mouse IgG2B Clone # 310829
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Semaphorin 7A
    Accession # O75326
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Semaphorin 7A in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with PHA were stained with Mouse Anti-Human Semaphorin 7A APC‑conjugated Monoclonal Antibody (Catalog # FAB20681A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Semaphorin 7A
Semaphorin 7A (Sema7A, designated CD108, previously Sema K1 or Sema L), is an ~80 kDa membrane-anchored glycoprotein that is a member of the Semaphorin family of axon guidance molecules (1-4). On human erythrocytes, it is the John Milton Hagen (JMH) blood group antigen (4). Sema7A is the only known Class 7 or glycophosphatidylinositol (GPI)-linked semaphorin; its expression is concentrated in the brain, spleen and thymus (1-5). Human Sema7A cDNA encodes a 44 amino acid (aa) signal sequence, a 604 aa extracellular domain (ECD) including Sema and C2-type Ig-like domains, and an 18 aa propeptide/GPI membrane anchor signal sequence. Mature human Sema7A shares 89%, 89%, 88%, 86% and 90% aa identity with mouse, rat, canine, bovine and equine Sema7A, respectively. The Sema7A sema domain contains an RGD integrin interaction motif (4). Although it binds plexin-C1 in vitro and may be coexpressed with it, many of its activities depend on interaction with beta 1 integrins such as alpha 1 beta 1 (6-10). Sema7A signaling through the two receptors may cause opposing effects (8). Sema7A is an immune semaphorin, with expression and activity on CD4+CD8+ thymocytes, activated T cells, macrophages and microglia (2, 9-12). T cell-expressed Sema7A interacts with monocytic cells, stimulating their chemotaxis, production of pro-inflammatory cytokines, and dendritic differentiation (5, 6). However, on the T cells themselves, Sema7A downregulates TCR signaling by promoting TCR internalization, modulating T cell responses (9). In lung macrophages, Sema7A is induced by TGF-beta and participates in TGF-beta -induced lung fibrosis (12). Sema7A is also expressed on pre-osteoblasts and osteoclasts, where it promotes migration and fusion, respectively; on keratinocytes, where it promotes melanocyte spreading and dendricity; and on some neurons, for example, promoting axon outgrowth in the developing olfactory tract (8, 10, 13). 
  • References:
    1. Yazdani, U. and J.R. Terman (2006) Genome Biol. 7:211.
    2. Kikutani, H. et al. (2007) Adv. Immunol. 93:121.
    3. Sato, Y. and Takahashi H. (1998) Biochim. Biophys. Acta. 1443:419.
    4. Yamada, A. et al. (1999) J. Immunol. 162:4094.
    5. Holmes, S. et al. (2002) Scand. J. Immunol. 56:270.
    6. Suzuki, K. et al. (2007) Nature 446:680.
    7. Pasterkamp, R.J. et al. (2007) BMC Dev. Biol. 7:98.
    8. Scott, G.A. et al. (2007) J. Invest. Dermatol. 128:151.
    9. Czopik, A.K. et al. (2006) Immunity 24:591.
    10. Pasterkamp, R.J. et al. (2003) Nature 424:398.
    11. Mine, T. et al. (2000) Tissue Antigens 55:429.
    12. Kang, H.R. et al. (2007) J. Exp. Med. 204:1083.
    13. Delorme, G. et al. (2005) Biol. Cell 97:589.
  • Entrez Gene IDs:
    8482 (Human); 20361 (Mouse)
  • Alternate Names:
    CD108 antigen; CD108; CD108MGC126696; CDw108; H-SEMA-K1; H-Sema-L; JMH blood group antigen; JMH; John-Milton-Hargen human blood group Ag; MGC126692; sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin)7A (JMH blood group); sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin)7A; sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, 7A; Sema K1; Sema L; Sema7A; SEMAK1; SEMAL; SEMALJohn Milton Hagen blood group H-Sema K1; semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group); semaphorin K1; semaphorin L; semaphorin-7A; semaphorin-K1; semaphorin-L
Related Research Areas
Isotype Controls
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Mouse IgG2B APC-conjugated Antibody

Ctrl IC0041A 4  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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