Human Serpin A4/Kallistatin Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF1669
Product Details
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Human Serpin A4/Kallistatin Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Serpin A4/Kallistatin in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross‑reactivity with recombinant human (rh) Serpin A1, rhSerpin A3, rhSerpin A5, rhSerpin C1, rhSerpin D1, rhSerpin E1, rhSerpin E2, and rhSerpin F2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Serpin A4/Kallistatin
Gln21-Pro427
Accession # AAA59454
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Serpin A4/Kallistatin (Catalog # 1669-PI)

Human Serpin A4/Kallistatin Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human Serpin A4/Kallistatin Antibody (Catalog # MAB16691)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin A4/Kallistatin

Serpin A4 is a member of the Serpin superfamily of the serine protease inhibitors (1). Known also as Kallistatin or proteinase inhibitor 4, Serpin A4 has been shown to function as a specific inhibitor of tissue kallikrein and as an inhibitor of angiogenesis and tumor growth (2, 3).

References
  1. Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
  2. Chen, V.C. et al. (2000) J. Biol. Chem. 275:40371.
  3. Miao, R.Q. et al. (2002) Blood 100:3245.
Entrez Gene IDs
5267 (Human)
Alternate Names
antitrypsin), member 4; KAL; Kallistatin; KLST; KSTKallikrein inhibitor; member 4; PI-4; PI4Peptidase inhibitor 4; protease inhibitor 4 (kallistatin); serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase; Serpin A4; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin)

Product Datasheets

FAQs

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