Human Serpin C1/Antithrombin-III Quantikine ELISA Kit

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  • Assay Length
    3.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (25 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Citrate Plasma (10 uL), Urine (25 uL), Human Milk (10 uL)
  • Sensitivity
    0.097 ng/mL
  • Assay Range
    0.3 - 20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine, Human Milk)
  • Specificity
    Natural and recombinant free and complexed human Serpin C1.  This assay cross-reacts 1.4% with recombinant mouse Serpin C1.
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity observed with 1 or more species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC208 , Quantikine Immunoassay Control Set 876 for Human Serpin C1 - Please Inquire
Product Summary
The Quantikine Human Serpin C1/Antithrombin-III Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human Serpin C1 in cell culture supernates, serum, plasma, urine, and human milk. It contains NS0-expressed recombinant human Serpin C1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Serpin C1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Serpin C1.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine, Human Milk
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 1.95 6.37 12.2 1.84 5.82 11.8
Standard Deviation 0.077 0.224 0.303 0.079 0.432 0.778
CV% 3.9 3.5 2.5 4.3 7.4 6.6

Recovery

The recovery of human Serpin C1 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 93 83-108
Urine (n=4) 99 92-109
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Serpin C1 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
 Serpin C1/Antithrombin-III [HRP]
Product Datasheets

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Serpin C1/Antithrombin-III
The human serpin superfamily consists of at least 35 members that target not only serine proteases, but also selected cysteine proteases and non-protease proteins. Serpins bind the protease active site resulting in a major conformational rearrangement that traps the enzyme in a covalent acyl-enzyme intermediate. As protease inhibitors, serpins have an array of functions including regulating blood clotting, the complement pathway, extracellular matrix remodeling, and cell motility. They are also involved in activities that extend beyond their ability to inhibit proteases. For instance, they may also regulate blood pressure, angiogenesis, or act as storage/transport proteins.
  • Entrez Gene IDs:
    462 (Human); 11905 (Mouse)
  • Alternate Names:
    Antithrombin-III; AT3antithrombin-III; ATIII; ATIIIantithrombin III; MGC22579; serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 1; serine-cysteine proteinase inhibitor clade C member 1; Serpin C1; serpin peptidase inhibitor, clade C (antithrombin), member 1
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ELISA Controls
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Quantikine Immunoassay Control Set 876 for Human Serpin C1

Ctrl QC208
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Supplemental ELISA Products
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Quantikine ELISA Wash Buffer 1

ELISA WA126 6  
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Average Rating: 5 (Based on 1 review)

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 Human Serpin C1/Antithrombin-III Quantikine ELISA Kit DSPC10 Array DSPC10
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  Anonymous 11/03/2016
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 Human Serpin C1/Antithrombin-III Quantikine ELISA Kit DSPC10
: Human Serpin C1/Antithrombin-III Quantikine ELISA Kit [DSPC10].

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