Human Serpin C1/Antithrombin-III Quantikine ELISA Kit

R&D Systems | Catalog # DSPC10

R&D Systems
100% Guarantee

Key Product Details

Assay Length

3.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (25 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Citrate Plasma (10 µL), Urine (25 µL), Human Milk (10 µL)

Sensitivity

0.097 ng/mL

Assay Range

0.313-20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine, Human Milk)
View all Serpin C1/Antithrombin-III ELISA Kits »

Product Summary for Human Serpin C1/Antithrombin-III Quantikine ELISA Kit

The Quantikine Human Serpin C1/Antithrombin-III Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human Serpin C1 in cell culture supernates, serum, plasma, urine, and human milk. It contains NS0-expressed recombinant human Serpin C1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Serpin C1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Serpin C1.

Product Specifications

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant free and complexed human Serpin C1.  This assay cross-reacts 1.4% with recombinant mouse Serpin C1.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity observed with 1 or more species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.

Cell Culture Supernates, Citrate Plasma, EDTA Plasma, Heparin Plasma, Human Milk, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 1.95 6.37 12.2 1.84 5.82 11.8
Standard Deviation 0.077 0.224 0.303 0.079 0.432 0.778
CV% 3.9 3.5 2.5 4.3 7.4 6.6

Recovery for Human Serpin C1/Antithrombin-III Quantikine ELISA Kit

The recovery of human Serpin C1 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 93 83-108
Urine (n=4) 99 92-109

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Serpin C1 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human Serpin C1/Antithrombin-III ELISA Linearity

Scientific Data Images for Human Serpin C1/Antithrombin-III Quantikine ELISA Kit

Human Serpin C1/Antithrombin-III ELISA Standard Curve

Human Serpin C1/Antithrombin-III ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Serpin C1/Antithrombin-III

The human serpin superfamily consists of at least 35 members that target not only serine proteases, but also selected cysteine proteases and non-protease proteins. Serpins bind the protease active site resulting in a major conformational rearrangement that traps the enzyme in a covalent acyl-enzyme intermediate. As protease inhibitors, serpins have an array of functions including regulating blood clotting, the complement pathway, extracellular matrix remodeling, and cell motility. They are also involved in activities that extend beyond their ability to inhibit proteases. For instance, they may also regulate blood pressure, angiogenesis, or act as storage/transport proteins.

Alternate Names

Antithrombin-III, ATIII

Entrez Gene IDs

462 (Human); 11905 (Mouse)

Gene Symbol

SERPINC1

Additional Serpin C1/Antithrombin-III Products

Product Documents for Human Serpin C1/Antithrombin-III Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Serpin C1/Antithrombin-III Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human Serpin C1/Antithrombin-III Quantikine ELISA Kit

Customer Reviews for Human Serpin C1/Antithrombin-III Quantikine ELISA Kit (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human Serpin C1/Antithrombin-III Quantikine ELISA Kit?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Human Serpin C1/Antithrombin-III Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Human Plasma
    Verified Customer | Posted 11/03/2016
    Human Serpin C1/Antithrombin-III Quantikine ELISA Kit DSPC10

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for ELISA Kits

Associated Pathways

Blood Coagulation Signaling Pathways Blood Coagulation Signaling Pathway Thumbnail