Human Serpin C1/Antithrombin-III Quantikine ELISA Kit

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DSPC10
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Human Serpin C1/Antithrombin-III ELISA Standard Curve
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Product Details
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Human Serpin C1/Antithrombin-III Quantikine ELISA Kit Summary

Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (25 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Citrate Plasma (10 uL), Urine (25 uL), Human Milk (10 uL)
Sensitivity
0.097 ng/mL
Assay Range
0.3 - 20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine, Human Milk)
Specificity
Natural and recombinant free and complexed human Serpin C1.  This assay cross-reacts 1.4% with recombinant mouse Serpin C1.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity observed with 1 or more species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Serpin C1/Antithrombin-III Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human Serpin C1 in cell culture supernates, serum, plasma, urine, and human milk. It contains NS0-expressed recombinant human Serpin C1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Serpin C1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Serpin C1.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine, Human Milk

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 1.95 6.37 12.2 1.84 5.82 11.8
Standard Deviation 0.077 0.224 0.303 0.079 0.432 0.778
CV% 3.9 3.5 2.5 4.3 7.4 6.6

Recovery

The recovery of human Serpin C1 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 93 83-108
Urine (n=4) 99 92-109

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Serpin C1 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Serpin C1/Antithrombin-III ELISA Linearity

Data Examples

Human Serpin C1/Antithrombin-III ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Serpin C1/Antithrombin-III

The human serpin superfamily consists of at least 35 members that target not only serine proteases, but also selected cysteine proteases and non-protease proteins. Serpins bind the protease active site resulting in a major conformational rearrangement that traps the enzyme in a covalent acyl-enzyme intermediate. As protease inhibitors, serpins have an array of functions including regulating blood clotting, the complement pathway, extracellular matrix remodeling, and cell motility. They are also involved in activities that extend beyond their ability to inhibit proteases. For instance, they may also regulate blood pressure, angiogenesis, or act as storage/transport proteins.

Entrez Gene IDs:
462 (Human); 11905 (Mouse)
Alternate Names:
Antithrombin-III; AT3antithrombin-III; ATIII; ATIIIantithrombin III; MGC22579; serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 1; serine-cysteine proteinase inhibitor clade C member 1; Serpin C1; serpin peptidase inhibitor, clade C (antithrombin), member 1
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

FAQs

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Human Serpin C1/Antithrombin-III Quantikine ELISA Kit
By Anonymous on 11/03/2016
Application: Sample Tested: Human plasma