Human Serpin C1/Antithrombin-III Quantikine ELISA Kit Summary
Sample Type & Volume Required Per Well
Cell Culture Supernates (25 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Citrate Plasma (10 uL), Urine (25 uL), Human Milk (10 uL)
Assay Range
0.3 - 20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine, Human Milk)
Specificity
Natural and recombinant free and complexed human Serpin C1. This assay cross-reacts 1.4% with recombinant mouse Serpin C1.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity observed with 1 or more species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human Serpin C1/Antithrombin-III Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human Serpin C1 in cell culture supernates, serum, plasma, urine, and human milk. It contains NS0-expressed recombinant human Serpin C1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Serpin C1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Serpin C1.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine, Human Milk
|
Intra-Assay Precision |
Inter-Assay Precision |
| Sample |
1 |
2 |
3 |
1 |
2 |
3 |
| n |
20 |
20 |
20 |
20 |
20 |
20 |
| Mean |
1.95 |
6.37 |
12.2 |
1.84 |
5.82 |
11.8 |
| Standard Deviation |
0.077 |
0.224 |
0.303 |
0.079 |
0.432 |
0.778 |
| CV% |
3.9 |
3.5 |
2.5 |
4.3 |
7.4 |
6.6 |
Recovery
The recovery of human Serpin C1 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type |
Average % Recovery |
Range % |
| Cell Culture Media (n=4) |
93 |
83-108 |
| Urine (n=4) |
99 |
92-109 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Serpin C1 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Data Examples
Human Serpin C1/Antithrombin-III ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Serpin C1/Antithrombin-III
The human serpin superfamily consists of at least 35 members that target not only serine proteases, but also selected cysteine proteases and non-protease proteins. Serpins bind the protease active site resulting in a major conformational rearrangement that traps the enzyme in a covalent acyl-enzyme intermediate. As protease inhibitors, serpins have an array of functions including regulating blood clotting, the complement pathway, extracellular matrix remodeling, and cell motility. They are also involved in activities that extend beyond their ability to inhibit proteases. For instance, they may also regulate blood pressure, angiogenesis, or act as storage/transport proteins.
Entrez Gene IDs:
462 (Human); 11905 (Mouse)
Alternate Names:
Antithrombin-III; AT3antithrombin-III; ATIII; ATIIIantithrombin III; MGC22579; serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 1; serine-cysteine proteinase inhibitor clade C member 1; Serpin C1; serpin peptidase inhibitor, clade C (antithrombin), member 1
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.
