Human Serum Albumin Antibody (JF32-10)
Novus Biologicals | Catalog # NBP2-66894
Recombinant Monoclonal Antibody
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Key Product Details
Species Reactivity
Human, Mouse, Rat, Bovine
Applications
Multiplex Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JF32-10 expressed in HEK293
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Product Specifications
Immunogen
Synthetic peptide within Human Human Serum Albumin aa 156-189 / 609. (SwissProt: P02768 Human; SwissProt: P07724 Mouse; SwissProt: P02770 Rat; SwissProt: P02769 Cow)
Localization
Secreted.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human Serum Albumin Antibody (JF32-10)
Western Blot: Human Serum Albumin Antibody (JF32-10) [NBP2-66894]
Western Blot: Human Serum Albumin Antibody (JF32-10) [NBP2-66894] - Western blot analysis of Human Serum Albumin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: HepG2 cell lysateImmunohistochemistry-Paraffin: Human Serum Albumin Antibody (JF32-10) [NBP2-66894]
Immunohistochemistry-Paraffin: Human Serum Albumin Antibody (JF32-10) [NBP2-66894] - Analysis of paraffin-embedded mouse lung tissue using anti-Albumin antibody. Counter stained with hematoxylin.Immunohistochemistry-Paraffin: Human Serum Albumin Antibody (JF32-10) [NBP2-66894]
Immunohistochemistry-Paraffin: Human Serum Albumin Antibody (JF32-10) [NBP2-66894] - Analysis of paraffin-embedded human kidney tissue using anti-Albumin antibody. Counter stained with hematoxylin.Immunohistochemistry-Paraffin: Human Serum Albumin Antibody (JF32-10) [NBP2-66894]
Immunohistochemistry-Paraffin: Human Serum Albumin Antibody (JF32-10) [NBP2-66894] - Analysis of paraffin-embedded human liver tissue using anti-Albumin antibody. Counter stained with hematoxylin.Immunohistochemistry-Paraffin: Human Serum Albumin Antibody (JF32-10) [NBP2-66894]
Immunohistochemistry-Paraffin: Human Serum Albumin Antibody (JF32-10) [NBP2-66894] - Analysis of paraffin-embedded human lung tissue using anti-Albumin antibody. Counter stained with hematoxylin.Immunohistochemistry-Paraffin: Human Serum Albumin Antibody (JF32-10) [NBP2-66894]
Immunohistochemistry-Paraffin: Human Serum Albumin Antibody (JF32-10) [NBP2-66894] - Analysis of paraffin-embedded human spleen tissue using anti-Albumin antibody. Counter stained with hematoxylin.Immunohistochemistry-Paraffin: Human Serum Albumin Antibody (JF32-10) [NBP2-66894]
Immunohistochemistry-Paraffin: Human Serum Albumin Antibody (JF32-10) [NBP2-66894] - Analysis of paraffin-embedded mouse liver tissue using anti-Albumin antibody. Counter stained with hematoxylin.Applications for Human Serum Albumin Antibody (JF32-10)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Human Serum Albumin
Alternate Names
albumin, cell growth inhibiting protein 42, DKFZp779N1935, growth-inhibiting protein 20, PRO0883, PRO0903, PRO1341, serum albumin
Gene Symbol
ALB
Additional Human Serum Albumin Products
Product Documents for Human Serum Albumin Antibody (JF32-10)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Human Serum Albumin Antibody (JF32-10)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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