Human SHP‑1 Alexa Fluor™ Plus 680‑conjugated Antibody

R&D Systems | Catalog # FAB1878AFP680

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Alexa Fluor Plus 680 (Excitation = 687 nm, Emission = 704 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 255402
View all SHP-1 Primary Antibodies »

Product Specifications

Specificity

Detects human SHP-1.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Human SHP‑1 Alexa Fluor™ Plus 680‑conjugated Antibody

Application
Recommended Usage

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: SHP-1

Src-Homology 2 domain Phosphatase-1 (SHP-1), also known as Protein Tyrosine Phosphatase 1C (PTP1C), PTPN6, and Hematopoetic Cell Phosphatase (HCPH), is an enzyme that selectively dephosphorylates tyrosine residues in proteins. Spontaneous point mutations in the SHP-1 gene in mice produce the “motheaten” and "motheaten viable” phenotypes that are severely autoimmune and immunodeficient (1). The enzyme is highly expressed in leukocyte cell types (2). SHP-1 has a regulatory region containing two Src homology 2 (SH2) domains that are critical for its binding to ITIM domains in inhibitory immunoreceptors (3). Deletion of the SH2 domains, as in this product, causes a marked increase in phosphatase activity (4). SHP-1 will dephosphorylate a wide variety of proteins, including the EGF receptor (5). A phosphopeptide containing the EGFR (Y992) sequence (R&D Systems, Catalog # ES006) can be used to measure the activity of SHP-1 by detecting the release of phosphate (R&D Systems, Catalog # DY996).

References

  1. Tsui, H.W. et al. (1993) Nature Genet. 4:124.
  2. Matthews, R.J. et al. (1992) Mol. Cell. Biol. 12:2396.
  3. Burshtyn, D.N. et al. (1997) J. Biol. Chem. 272:13066.
  4. Pei, D. et al. (1994) Biochemistry 33:15483.
  5. Tomic, S. et al. (1995) J. Biol. Chem. 270:21277.

Long Name

Src Homology Region 2 Domain Phosphatase 1

Alternate Names

HCPH, PTP1C, PTPN6, SHP1

Entrez Gene IDs

5777 (Human); 15170 (Mouse); 116689 (Rat)

Gene Symbol

PTPN6

UniProt

P29350

Additional SHP-1 Products

Product Documents for Human SHP‑1 Alexa Fluor™ Plus 680‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human SHP‑1 Alexa Fluor™ Plus 680‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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