Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry

Cited:

Immunohistochemistry, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 255402
View all SHP-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human SHP-1
Ala205-Lys595
Accession # P29350

Specificity

Detects human SHP-1.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human SHP‑1 Antibody

SHP-1 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

SHP‑1 in HeLa Human Cell Line.

SHP-1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rat Anti-Human SHP-1 Monoclonal Antibody (Catalog # MAB1878) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human SHP‑1 Antibody

Application
Recommended Usage

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells and HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SHP-1

Src-Homology 2 domain Phosphatase-1 (SHP-1), also known as Protein Tyrosine Phosphatase 1C (PTP1C), PTPN6, and Hematopoetic Cell Phosphatase (HCPH), is an enzyme that selectively dephosphorylates tyrosine residues in proteins. Spontaneous point mutations in the SHP-1 gene in mice produce the “motheaten” and "motheaten viable” phenotypes that are severely autoimmune and immunodeficient (1). The enzyme is highly expressed in leukocyte cell types (2). SHP-1 has a regulatory region containing two Src homology 2 (SH2) domains that are critical for its binding to ITIM domains in inhibitory immunoreceptors (3). Deletion of the SH2 domains, as in this product, causes a marked increase in phosphatase activity (4). SHP-1 will dephosphorylate a wide variety of proteins, including the EGF receptor (5). A phosphopeptide containing the EGFR (Y992) sequence (R&D Systems, Catalog # ES006) can be used to measure the activity of SHP-1 by detecting the release of phosphate (R&D Systems, Catalog # DY996).

References

  1. Tsui, H.W. et al. (1993) Nature Genet. 4:124.
  2. Matthews, R.J. et al. (1992) Mol. Cell. Biol. 12:2396.
  3. Burshtyn, D.N. et al. (1997) J. Biol. Chem. 272:13066.
  4. Pei, D. et al. (1994) Biochemistry 33:15483.
  5. Tomic, S. et al. (1995) J. Biol. Chem. 270:21277.

Long Name

Src Homology Region 2 Domain Phosphatase 1

Alternate Names

HCPH, PTP1C, PTPN6, SHP1

Entrez Gene IDs

5777 (Human); 15170 (Mouse); 116689 (Rat)

Gene Symbol

PTPN6

UniProt

P29350

Additional SHP-1 Products

Product Documents for Human SHP‑1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human SHP‑1 Antibody

For research use only

Citations for Human SHP‑1 Antibody

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