Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Flow Cytometry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # 908102
View all Siglec-1/CD169 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Siglec‑1/CD169
Ser20-Gln1641
Accession # Q9BZZ2

Specificity

Detects human Siglec‑1/CD169 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Siglec‑1/CD169 Alexa Fluor® 488‑conjugated Antibody

Detection of Siglec‑1/CD169 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of Siglec‑1/CD169 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs), (A) resting, or (B) treated with 5 ng/mL Recombinant Human IFN-a (Catalog # 11100-1) overnight, were stained with Mouse Anti-Human Siglec-1/CD169 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB5197G) and Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A). Quadrant markers were set based on control antibody staining (Catalog # IC002G). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Siglec‑1/CD169 Alexa Fluor® 488‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

5 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with Recombinant Human IFN‑a (Catalog # 11100-1)

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Siglec-1/CD169

Siglecs are sialic acid specific I-type lectins that belong to the immunoglobulin superfamily. Structurally, they are transmembrane proteins with an N-terminal Ig-like V‑set domain followed by varying numbers of Ig-like C2-set domains (1, 2). Human Siglec-1, also known as sialoadhesin and CD169, is a 175‑185 kDa glycoprotein. It contains a 1622 amino acid (aa) extracellular domain (ECD) with one Ig-like V‑set domain and 16 Ig-like C2-set domains, a 21 aa transmembrane segment, and a 47 aa cytoplasmic domain (3). Within the ECD, human Siglec-1 shares approximately 70% aa sequence identity with mouse and rat Siglec-1. Alternate splicing generates a potentially soluble form of the ECD, while a second isoform shows a substituted cytoplasmic domain. Siglec-1 expression occurs on monocytes, dendritic cells, and macrophages of various organs and tissues, including liver, spleen, lung, and gut. The adhesive function of Siglec-1 is supported by the N-terminal Ig-like domain which shows a selectivity for alpha 2,3‑linked sialic acid residues (3‑5). Siglec-1 binds a number of sialylated molecules including the mannose receptor, MGL1, MUC1, PSGL-1, and different glycoforms of CD43 (6‑9). Its binding capacity can be masked by endogenous sialylated molecules (10, 11). The sialylated and sulfated N-linked carbohydrates that modify Siglec-1 itself are required for ligand binding (6, 7). Siglec-1 is expressed on dendritic cells following rhinovirus exposure, and these DC promote T cell anergy (12). It is also induced on circulating monocytes during systemic sclerosis and HIV-1 infection (13‑15). Siglec-1 can trap HIV-1 particles for trans infection of permissive cells (14).

References

  1. Varki, A. and T. Angata (2006) Glycobiology 16:1R.
  2. Crocker, P.R. et al. (2007) Nat. Rev. Immunol. 7:255.
  3. Hartnell, A. et al. (2001) Blood 97:288.
  4. Nath, D. et al. (1995) J. Biol. Chem. 270:26184.
  5. Crocker, P.R. et al. (1991) EMBO J. 10:1661.
  6. Martinez-Pomares, L. et al. (1999) J. Biol. Chem. 274:35211.
  7. Kumamoto, Y. et al. (2004) J. Biol. Chem. 279:49274.
  8. Nath, D. et al. (1999) Immunology 98:213.
  9. van den Berg, T.K. et al. (2001) J. Immunol. 166:3637.
  10. Nakamura, K. et al. (2002) Glycobiology 12:209.
  11. Barnes, Y.C. et al. (1999) Blood 93:1245.
  12. Kirchberger, S. et al. (2005) J. Immunol. 175:1145.
  13. York, M.R. et al. (2007) Arthritis Rheum. 56:1010.
  14. Rempel, H. et al. (2008) PloS ONE 3:e1967.
  15. van der Kuyl, A.C. et al. (2007) Plos ONE 2:e257.

Long Name

Sialic Acid Binding Ig-like Lectin 1

Alternate Names

CD169, Siglec1

Entrez Gene IDs

6614 (Human); 20612 (Mouse); 311426 (Rat)

Gene Symbol

SIGLEC1

UniProt

Q9BZZ2 (Human)

Additional Siglec-1/CD169 Products

Product Documents for Human Siglec‑1/CD169 Alexa Fluor® 488‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human Siglec‑1/CD169 Alexa Fluor® 488‑conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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