Detects human Siglec-11 in direct ELISAs and Western blots. In direct ELISA and Western blots, approximately 50% cross-reactivity with recombinant human (rh) Siglec-10 is observed and less than 5% cross-reactivity with rhSiglec-2, -3, -5, -6, -7, -9 and recombinant mouse Siglec-F is observed.
Polyclonal Goat IgG
Chinese hamster ovary cell line CHO-derived recombinant human Siglec-11 Asn17-His543 (Glu84Ala, Lys145Gln) Accession # Q96RL6
Supplied in a saline solution containing BSA and Sodium Azide.
Detection of Siglec‑11 in Human Peripheral Blood Mononuclear Cell Monocytes and Granulocytes by Flow Cytometry.
Human peripheral blood mononuclear cell monocytes and granulocytes were stained with Mouse Anti-Human CD14 APC‑conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Goat Anti-Human Siglec‑11 PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB3258P) or (B) Normal Goat IgG Phycoerythrin Control (Catalog # IC108P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Siglecs (Sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N‑terminal Ig-like V-set domain which mediates sialic acid binding, followed by a varying numbers of Ig-like C2-set domains. Siglecs‑3 and -5 through -13 constitute the CD33/Siglec-3 related group, which are defined by their sequence homology and differential expression in the hematopoietic system. Mature human Siglec-11 is an 85-90 kDa, type I transmembrane glycoprotein that consists of a 534 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 114 aa cytoplasmic domain. The ECD contains one Ig-like V-set domain, and three Ig-like C2-set domains. The cytoplasmic domain contains two Immunoreceptor Tyrosine-based Inhibitory Motifs (ITIMs). A splice variant of Siglec-11 has a deletion of nearly 100 aa in the extracellular juxtamembrane region. Among siglecs, the ECD of Siglec-11 is most closely related to that of Siglec-10 (82% aa sequence identity). The cytoplasmic domains of these proteins are only 20% identical. Siglec-11 is closely related to the pseudogenes Siglec-14 and Siglec-16. Human Siglec-11 shares 90%‑96% aa sequence identity with Siglec-11 from great apes. Rodent orthologs of Siglec-11 have not been identified. In human, Siglec-11 is expressed in tissue macrophages, brain microglia, and inflammatory site monocytes. Strong microglial expression is specific to humans, as it is less prominent or absent in chimpanzees and orangutans. Siglec-11 forms 180 kDa disulfide-linked dimers. It shows a strong binding preference for sialic acid in alpha 2-8 linkage which is unusual for siglecs. Notably, this applies to polysialyated NCAM residues on neurons where Siglec-11 activation imparts neuroprotection towards neurons in response to neuroinflammation.
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