Human Siglec‑3/CD33 (Research Grade Gemtuzumab Biosimilar) Antibody

R&D Systems | Catalog # MAB9635

Recombinant Monoclonal Antibody For Research Use Only
R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, CyTOF-ready

Cited:

Neutralization

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # Hu9a
View all Siglec-3/CD33 Primary Antibodies »

Product Specifications

Immunogen

Human Siglec‑3/CD33

Specificity

Detects human Siglec‑3/CD33 based on Gemtuzumab therapeutic antibody. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Gemtuzumab. This product is for research use only.

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human Siglec‑3/CD33 (Research Grade Gemtuzumab Biosimilar) Antibody

Detection of Siglec-3/CD33 on U937 Human Cell Line antibody by Flow Cytometry.

Detection of Siglec-3/CD33 on U937 Human Cell Line by Flow Cytometry.

U937 Human histiocytic lymphoma cell line was stained with Human Anti-Human Siglec-3/CD33 (Research Grade Gemtuzumab Biosimilar) Monoclonal Antibody (Catalog # MAB9635, filled histogram) or irrelevant antibody (open histogram) followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Siglec‑3/CD33 (Research Grade Gemtuzumab Biosimilar) Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: U937 Human Histiocytic Lymphoma cell line

Reviewed Applications

Read 1 review rated 5 using MAB9635 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Siglec-3/CD33

Long Name

Sialic Acid Binding Ig-like Lectin 3

Alternate Names

CD33, gp67, Siglec3

Entrez Gene IDs

945 (Human); 12489 (Mouse); 102117580 (Cynomolgus Monkey)

Gene Symbol

CD33

Additional Siglec-3/CD33 Products

Product Documents for Human Siglec‑3/CD33 (Research Grade Gemtuzumab Biosimilar) Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Siglec‑3/CD33 (Research Grade Gemtuzumab Biosimilar) Antibody

For research use only

Customer Reviews for Human Siglec‑3/CD33 (Research Grade Gemtuzumab Biosimilar) Antibody (1)

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Customer Images

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  • Human Siglec-3/CD33 (Research Grade Gemtuzumab Biosimilar) Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Recombinant protein
    Species: Human
    Verified Customer | Posted 10/03/2025
    Human Siglec‑3/CD33 (Research Grade Gemtuzumab Biosimilar) Antibody MAB9635
    Bio-Techne Response
    This review reflects a new species or application tested on a primary antibody.

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FAQs for Human Siglec‑3/CD33 (Research Grade Gemtuzumab Biosimilar) Antibody

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  • Q: Does this Biosimilar antibody have the same sequence as the therapeutic antibody?

    A: The Biosimilar antibody has the same variable region and constant region sequence as the therapeutic antibody, except for Catalog numbers MAB9677, MAB9575, MAB9577, MAB9589, and their conjugates. These four antibodies have the same variable region as the Biosimilar, but not the same constant region.

  • Q: What is a Biosimilar antibody?

    A: A Biosimilar antibody is a non-therpeutic, recombinantly-expressed antibody which has the same sequence as the corresponding therapeutic antibody.  It is for research use only. The nomenclature of the Biosimilar antibody includes the name of the therapeutic antibody.  For example, Catalog # MAB9577 is called Human EGFR (Research Grade CetuxiMab Biosimilar) antibody.

  • Q: Does this Biosimilar antibody have the same sequence as the therapeutic antibody?

    A: The Biosimilar antibody has the same variable region and constant region sequence as the therapeutic antibody, except for Catalog numbers MAB9677, MAB9575, MAB9577, MAB9589, and their conjugates. These four antibodies have the same variable region as the Biosimilar, but not the same constant region.

  • Q: What is a Biosimilar antibody?

    A: A Biosimilar antibody is a non-therpeutic, recombinantly-expressed antibody which has the same sequence as the corresponding therapeutic antibody.  It is for research use only. The nomenclature of the Biosimilar antibody includes the name of the therapeutic antibody.  For example, Catalog # MAB9577 is called Human EGFR (Research Grade CetuxiMab Biosimilar) antibody.

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