Human Siglec‑3/CD33 (Research Grade Gemtuzumab Biosimilar) Biotinylated Antibody

R&D Systems | Catalog # FAB9635B

Recombinant Monoclonal Antibody For Research Use Only
R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Biotin

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # Hu9a
View all Siglec-3/CD33 Primary Antibodies »

Product Specifications

Immunogen

Human Siglec‑3/CD33

Specificity

Detects human Siglec‑3/CD33 based on Gemtuzumab therapeutic antibody. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Gemtuzumab. This product is for research use only.

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Scientific Data Images

Detection of Siglec-3/CD33 antibody in U937 human histiocytic lymphoma cell line antibody by Flow Cytometry.

Detection of Siglec-3/CD33 in U937 human histiocytic lymphoma cell line by Flow Cytometry.

U937 human histiocytic lymphoma cell line was stained with biotinylated Human Anti-Human Siglec-3/CD33 (Research Grade Gemtuzumab Biosimilar) Monoclonal Antibody (Catalog # FAB9635B, filled histogram) or irrelevant biotinylated antibody (open histogram) followed by APC-conjugated Streptavidin (Catalog # F0050). View our protocol for Staining Membrane-associated Proteins.

Applications

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: U937 human histiocytic lymphoma cell line

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Siglec-3/CD33

Gemtuzumab is a biosimilar directed against the extracellular domain of CD33. This biotinylated antibody can be used in flow cytometry or various assay format to measure Siglec-3/CD33 expression and can be detected by conjugated streptavidin.

Long Name

Sialic Acid Binding Ig-like Lectin 3

Alternate Names

CD33, gp67, Siglec3

Entrez Gene IDs

945 (Human); 12489 (Mouse); 102117580 (Cynomolgus Monkey)

Gene Symbol

CD33

Additional Siglec-3/CD33 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices

For research use only

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