Human Siglec-3/CD33 (Research Grade Gemtuzumab Biosimilar) Biotinylated Antibody

Catalog #: FAB9635B Datasheet / COA / SDS

Recombinant Monoclonal Antibody For Research Use Only

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FAB9635B-100

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Detection of Siglec-3/CD33 in U937 human histiocytic lymphoma cell line by Flow Cytometry.

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Human Siglec-3/CD33 (Research Grade Gemtuzumab Biosimilar) Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Siglec‑3/CD33 based on Gemtuzumab therapeutic antibody. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Gemtuzumab. This product is for research use only.

Source

Recombinant Monoclonal Human IgG₁ Clone # Hu9a

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Human Siglec‑3/CD33

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Biotin

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Siglec-3/CD33 in U937 human histiocytic lymphoma cell line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with biotinylated Human Anti-Human Siglec-3/CD33 (Research Grade Gemtuzumab Biosimilar) Monoclonal Antibody (Catalog # FAB9635B, filled histogram) or irrelevant biotinylated antibody (open histogram) followed by APC-conjugated Streptavidin (Catalog # F0050). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Siglec-3/CD33

Gemtuzumab is a biosimilar directed against the extracellular domain of CD33. This biotinylated antibody can be used in flow cytometry or various assay format to measure Siglec-3/CD33 expression and can be detected by conjugated streptavidin.

Long Name

Sialic Acid Binding Ig-like Lectin 3

Entrez Gene IDs

945 (Human); 12489 (Mouse); 102117580 (Cynomolgus Monkey)

Alternate Names

CD33 antigen (gp67); CD33 antigen; CD33 molecule; CD33; FLJ00391; gp67; myeloid cell surface antigen CD33; p67; sialic acid binding Ig-like lectin 3; Sialic acid-binding Ig-like lectin 3; Siglec3; Siglec-3; SIGLEC3gp67