Siglecs (sialic acid binding Ig-like lectins) are a subgroup of the immunoglobulin superfamily that interact with sialic acids in glycoproteins and glycolipids. Siglec-5 binds to alpha-2, 3- and alpha-2, 6-linked sialic acid equally. It occurs as a disulfide-linked dimer of approximately 140 kDa with the highest expression levels in hematopoietic tissues. Siglec-5 has an inhibitory motif within its cytoplasmic domain. Siglec-14 is an activating receptor that shares 99.5% aa sequence identity with Siglec-5 through the first two extracellular Ig domains and displays a similar glycan binding preference.
Human Siglec‑5/Siglec‑14 Biotinylated Antibody
R&D Systems | Catalog # BAM10722
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Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA Detection (Matched Antibody Pair)
Cited:
Immunocytochemistry
Label
Biotin
Antibody Source
Monoclonal Mouse IgG1 Clone # 194111
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Siglec‑5/Siglec‑14
Lys18-Thr434
Accession # O15389
Lys18-Thr434
Accession # O15389
Specificity
Detects human Siglec‑5/Siglec‑14 in ELISAs. In sandwich immunoassays, 100% cross-reactivity with recombinant human (rh) Siglec-14 is observed and no cross-reactivity with rhSiglec-3, rhSiglec-7, or rhSiglec-9 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Human Siglec‑5/Siglec‑14 Biotinylated Antibody
Human Siglec-5 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Siglec-5/Siglec-14
Long Name
Sialic Acid Binding Ig-like Lectin 5
Alternate Names
CD170;CD33L2;OBBP2;OB-BP2;Sialic acid-binding Ig-like lectin 5;SIGLEC5;SIGLEC-5
Entrez Gene IDs
8778 (Human)
Gene Symbol
SIGLEC5
UniProt
Additional Siglec-5/Siglec-14 Products
Product Documents for Human Siglec‑5/Siglec‑14 Biotinylated Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Siglec‑5/Siglec‑14 Biotinylated Antibody
For research use only
Citations for Human Siglec‑5/Siglec‑14 Biotinylated Antibody
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