|Cell Adhesion Mediated by Siglec‑5 and Neutralization by Human Siglec‑5/14 Antibody. Recombinant Human Siglec‑5 Fc Chimera (Catalog # 1072-SL), immobilized onto a microplate, supports the adhesion of human red blood cells in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human Siglec‑5 Fc Chimera (5 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Siglec‑5/14 Monoclonal Antibody (Catalog # MAB10721). The ND50 is typically 0.04-0.2 µg/mL.|
|Detection of Siglec‑5/14 in Human Monocytes by Flow Cytometry. Human blood derived monocytes were stained with Mouse Anti-Human Siglec‑5/14 Monoclonal Antibody (Catalog # MAB10721, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).|
Siglecs (sialic acid binding Ig-like lectins) are a subgroup of the immunoglobulin superfamily that interact with sialic acids in glycoproteins and glycolipids. Siglec-5 binds to alpha-2, 3- and alpha-2, 6-linked sialic acid equally. It occurs as a disulfide-linked dimer of approximately 140 kDa with the highest expression levels in hematopoietic tissues. Siglec-5 has an inhibitory motif within its cytoplasmic domain. Siglec-14 is an activating receptor that shares 99.5% aa sequence identity with Siglec-5 through the first two extracellular Ig domains and displays a similar glycan binding preference.
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