Human Siglec-6/CD327 Alexa Fluor® 700-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB2859N-025
FAB2859N-100
Human Siglec-6/CD327 Alexa Fluor® 700-conjugated Antibody in Flow Cytometry
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Human Siglec-6/CD327 Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Siglec‑6/CD327 in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 767329
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Siglec‑6/CD327
Gln27-Val331
Accession # NP_942142
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 700

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of Siglec‑6/CD327 in U937 Human Cell Line by Flow Cytometry. View Larger

Detection of Siglec‑6/CD327 in U937 Human Cell Line by Flow Cytometry.
U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human
Siglec‑6/CD327 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB2859N, filled histogram) or isotype control antibody (open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of Siglec‑6/CD327 in Human PBMCs by Flow Cytometry. View Larger

Detection of Siglec‑6/CD327 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 PE‑conjugated Monoclonal Antibody (Catalog # FAB4867P) and either (A) Mouse Anti-Human Siglec‑6/CD327 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB2859N) or (B) Mouse Alexa Fluor 700 Isotype Control. View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Siglec-6/CD327

Siglecs (Sialic acid binding Ig-like Lectins) are I-type (Ig-type) lectins that belong to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains (1-4). Eleven human Siglecs (Siglec-1 through 11) have been cloned and characterized. Within these eleven, there are at least two groups, one of which is termed the CD33-related group. CD33-related Siglecs include CD33/Siglec-3 and Siglec-5 through 11 (1, 3). To date, no Siglec has been shown to recognize any cell surface ligand other than sialic acid. This suggests that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. The cDNA of human Siglec-6 (also known as OB-BP1 and CD33L), encodes a putative 442 amino acid (aa) protein that contains a 15 aa signal peptide, a 321 aa extracellular region, a 21 aa transmembrane region (TM), and an 85 aa cytoplasmic tail (5, 6). The extracellular region contains one N-terminal V-type Ig-like domain followed by two Ig-like C2-type domains. The cytoplasmic domain has one immunoreceptor tyrosine-based inhibition motif (ITIM). At least three additional isoforms exist, all of which encode an additional 11 aa’s at the N-terminus, likely due to the utilization of an alternate start site. Two of the three isoforms also show splicing. One isoform shows a 16 aa in-frame deletion in the second C2-like domain, while the other shows a deletion of the TM and cytoplasmic region, thus potentially generating a soluble form (6-9). Siglec-6 is found on B cells and in placenta, and would seem to have a restricted specificity for the sialyl Tn antigen (6, 10).

References
  1. Crocker, P.R. and J. Zhang (2002) Biochem. Soc. Symp. 69:83.
  2. Crocker, P.R. and A. Varki (2001) Immunology 103:137.
  3. Crocker, P.R. (2002) Curr. Opin. Struct. Biol. 12:609.
  4. Powell, L.D. and A. Varki (1995) J. Biol. Chem. 270:14243.
  5. Takei, Y. et al. (1997) Cytogenet. Cell Genet. 78:295.
  6. Patel, N. et al. (1999) J. Biol. Chem. 274:22729.
  7. GenBank Accession # NP_942143.
  8. GenBank Accession # NP_942142.
  9. GenBank Accession # NP_001236.
  10. Crocker, P.R. and A. Varki (2001) Trends Immunol. 22:337.
Long Name
Sialic Acid Binding Ig-like Lectin 6
Entrez Gene IDs
946 (Human)
Alternate Names
CD327 antigen; CD327; CD33 antigen-like 1; CD33L1; CD33L1OBBP1CD327; CD33L2; CD33LCDW327; CDw327; OB-BP1; Obesity-binding protein 1; sialic acid binding Ig-like lectin 6; sialic acid-binding Ig-like lectin 6; Siglec6; Siglec-6

Product Datasheets

Citation for Human Siglec-6/CD327 Alexa Fluor® 700-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Lipopolysaccharide inhalation recruits monocytes and dendritic cell subsets to the alveolar airspace
    Authors: L Jardine, S Wiscombe, G Reynolds, D McDonald, A Fuller, K Green, A Filby, I Forrest, MH Ruchaud-Sp, J Scott, M Collin, M Haniffa, AJ Simpson
    Nat Commun, 2019;10(1):1999.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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