Human Siglec-6/CD327 APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human Siglec‑6/CD327 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG1 Clone # 767329
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human Siglec‑6/CD327
    Accession # NP_942142
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of Siglec‑6/CD327 in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human Siglec‑6/CD327 APC‑conjugated Monoclonal Antibody (Catalog # FAB2859A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of Siglec‑6/CD327 in Human PBMC lymphocytes by Flow Cytometry. Human peripheral blood mononuclear cell (PBMC) lymphocytes were stained with Mouse Anti-Human CD19 PE‑conjugated Monoclonal Antibody (Catalog # FAB4867P) and either (A) Mouse Anti-Human Siglec‑6/CD327 APC‑conjugated Monoclonal Antibody (Catalog # FAB2859A) or (B) Mouse IgG1 Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Siglec-6/CD327

Siglecs (Sialic acid binding Ig-like Lectins) are I-type (Ig-type) lectins that belong to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains (1-4). Eleven human Siglecs (Siglec-1 through 11) have been cloned and characterized. Within these eleven, there are at least two groups, one of which is termed the CD33-related group. CD33-related Siglecs include CD33/Siglec-3 and Siglec-5 through 11 (1, 3). To date, no Siglec has been shown to recognize any cell surface ligand other than sialic acid. This suggests that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. The cDNA of human Siglec-6 (also known as OB-BP1 and CD33L), encodes a putative 442 amino acid (aa) protein that contains a 15 aa signal peptide, a 321 aa extracellular region, a 21 aa transmembrane region (TM), and an 85 aa cytoplasmic tail (5, 6). The extracellular region contains one N-terminal V-type Ig-like domain followed by two Ig-like C2-type domains. The cytoplasmic domain has one immunoreceptor tyrosine-based inhibition motif (ITIM). At least three additional isoforms exist, all of which encode an additional 11 aa’s at the N-terminus, likely due to the utilization of an alternate start site. Two of the three isoforms also show splicing. One isoform shows a 16 aa in-frame deletion in the second C2-like domain, while the other shows a deletion of the TM and cytoplasmic region, thus potentially generating a soluble form (6-9). Siglec-6 is found on B cells and in placenta, and would seem to have a restricted specificity for the sialyl Tn antigen (6, 10).

  • References:
    1. Crocker, P.R. and J. Zhang (2002) Biochem. Soc. Symp. 69:83.
    2. Crocker, P.R. and A. Varki (2001) Immunology 103:137.
    3. Crocker, P.R. (2002) Curr. Opin. Struct. Biol. 12:609.
    4. Powell, L.D. and A. Varki (1995) J. Biol. Chem. 270:14243.
    5. Takei, Y. et al. (1997) Cytogenet. Cell Genet. 78:295.
    6. Patel, N. et al. (1999) J. Biol. Chem. 274:22729.
    7. GenBank Accession # NP_942143.
    8. GenBank Accession # NP_942142.
    9. GenBank Accession # NP_001236.
    10. Crocker, P.R. and A. Varki (2001) Trends Immunol. 22:337.
  • Long Name:
    Sialic Acid Binding Ig-like Lectin 6
  • Entrez Gene IDs:
    946 (Human)
  • Alternate Names:
    CD327 antigen; CD327; CD33 antigen-like 1; CD33L1; CD33L1OBBP1CD327; CD33L2; CD33LCDW327; CDw327; OB-BP1; Obesity-binding protein 1; sialic acid binding Ig-like lectin 6; sialic acid-binding Ig-like lectin 6; Siglec6; Siglec-6
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 APC-conjugated Antibody

Ctrl IC002A 5  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human Siglec-6/CD327 APC-conjugated Antibody?

Submit a review and receive a $25 amazon gift card if you include an image - $10 Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Certificate of Analysis Lookup

The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided

Certificate of Analysis Lookup

Research Areas of Interest
R&D Systems Guarantee


Customer Information