Human Siglec-8 Alexa Fluor® 700-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB7975N
Human Siglec-8 Alexa Fluor® 700-conjugated Antibody in Data
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Human Siglec-8 Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Siglec-8 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Siglec-7, -9, or -14 is observed.
Source
Monoclonal Mouse IgG1 Clone # 837535
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Siglec-8
Met17-Ala363
Accession # Q9NYZ4
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Siglec-8 in Human Blood Granulocytes by Flow Cytometry. View Larger

Detection of Siglec-8 in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Rat Anti-Human CCR3 PE-conjugated Monoclonal Antibody (Catalog # FAB155P) and either (A) Mouse Anti-Human Siglec-8 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB7975N) or (B) Mouse IgG1Alexa Fluor 700 Isotype Control (Catalog # IC002N). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Siglec-8

Siglec-8 (Sialic acid-binding Ig-like lectin 8), formerly called SAF-2 (sialoadhesin family member 2) and designated CD329, is a 75 kDa member of the inhibitory CD33‑related SIGLEC family within the Ig superfamily of proteins. An alternately spliced 48 kDa short form lacks most of the intracellular domain, while another isoform lacks the second of three extracellular Ig domains, representing amino acids (aa) 152-245 of the 499 aa long form. Siglec-8 is expressed exclusively on eosinophils, mast cells, and weakly on basophils, and is a marker for eosinophil terminal differentiation. It specifically recognizes 6’ sulfated sialyl Lewis X carbohydrate structures. Engagement results in eosinophils apoptosis, or mast cell inhibition of degranulation. Eosinophil Siglec F is the mouse paralog of human Siglec-8, but shares <40% amino acid sequence identity.

Long Name
Sialic Acid Binding Ig-like Lectin 8
Entrez Gene IDs
27181 (Human)
Alternate Names
CD329 antigen; CDw329; MGC59785; SAF2; SAF2SAF-2; sialic acid binding Ig-like lectin 8; Sialoadhesin family member 2; Siglec8; Siglec-8; SIGLEC8L; SIGLEC8Lsialic acid-binding Ig-like lectin 8

Product Datasheets

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Reviews for Human Siglec-8 Alexa Fluor® 700-conjugated Antibody

Average Rating: 5 (Based on 2 Reviews)

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Human Siglec-8 Alexa Fluor® 700-conjugated Antibody
By Anonymous on 05/15/2020
Application: Flow Sample Tested: Peripheral blood granulocytes Species: Human

I used this antibody to stain human whole blood samples. First CD45 and side scatter were used to identify the major leukocyte populations in blood. Gating on granulocytes was followed by plotting with CD66b for neutrophils and Siglec-8 for eosinophils. Eosinophils can be clearly identified by higher Siglec-8 expression compared to neutrophils, which are negative for this marker. Fixation and storage at 4 degrees does not result in substantial loss of signal. This antibody was also use to create single stained compensation control using beads, but voltage may need to be optimized to achieve sufficiently bright signal as the relative brightness for AF700 is quite low.


Human Siglec-8 Alexa Fluor® 700-conjugated Antibody
By Anonymous on 09/05/2019
Application: Flow Sample Tested: Peripheral blood leukocytes Species: Mouse

I used the AF700 anti-Siglec 8 to identify peripheral blood eosinophils in human CF patients. This antibody has good specificity in differentiating eosinophils from other granulocytes