Human Siglec-8 Alexa Fluor® 700-conjugated Antibody Summary
Accession # Q9NYZ4
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Siglec-8 in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Rat Anti-Human CCR3 PE-conjugated Monoclonal Antibody (Catalog # FAB155P) and either (A) Mouse Anti-Human Siglec-8 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB7975N) or (B) Mouse IgG1Alexa Fluor 700 Isotype Control (Catalog # IC002N). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Siglec-8 (Sialic acid-binding Ig-like lectin 8), formerly called SAF-2 (sialoadhesin family member 2) and designated CD329, is a 75 kDa member of the inhibitory CD33‑related SIGLEC family within the Ig superfamily of proteins. An alternately spliced 48 kDa short form lacks most of the intracellular domain, while another isoform lacks the second of three extracellular Ig domains, representing amino acids (aa) 152-245 of the 499 aa long form. Siglec-8 is expressed exclusively on eosinophils, mast cells, and weakly on basophils, and is a marker for eosinophil terminal differentiation. It specifically recognizes 6’ sulfated sialyl Lewis X carbohydrate structures. Engagement results in eosinophils apoptosis, or mast cell inhibition of degranulation. Eosinophil Siglec F is the mouse paralog of human Siglec-8, but shares <40% amino acid sequence identity.
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human Siglec-8 Alexa Fluor® 700-conjugated Antibody
Average Rating: 5 (Based on 2 Reviews)
Have you used Human Siglec-8 Alexa Fluor® 700-conjugated Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
I used this antibody to stain human whole blood samples. First CD45 and side scatter were used to identify the major leukocyte populations in blood. Gating on granulocytes was followed by plotting with CD66b for neutrophils and Siglec-8 for eosinophils. Eosinophils can be clearly identified by higher Siglec-8 expression compared to neutrophils, which are negative for this marker. Fixation and storage at 4 degrees does not result in substantial loss of signal. This antibody was also use to create single stained compensation control using beads, but voltage may need to be optimized to achieve sufficiently bright signal as the relative brightness for AF700 is quite low.
I used the AF700 anti-Siglec 8 to identify peripheral blood eosinophils in human CF patients. This antibody has good specificity in differentiating eosinophils from other granulocytes