Human CCR3 PE-conjugated Antibody

Clone 61828 was used by HLDA to establish CD designation.
(9 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human CCR3 transfectants in flow cytometry. In flow cytometry, no cross-reactivity with CCR1, CCR2, CCR5, and CCR6 transfectants or the parental cell line is observed. Detects CCR3 on peripheral blood eosinophils.
  • Source
    Monoclonal Rat IgG2A Clone # 61828
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Y3 rat myeloid cell line transfected with human CCR3
    Accession # P51677
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CCR3 in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human Integrin  alpha 4/CD49d APC‑conjugated Monoclonal Antibody (Catalog # FAB1354A) and either (A) Rat Anti-Human CCR3 PE‑conjugated Monoclonal Antibody (Catalog # FAB155P) or (B) Rat IgG2A Phycoerythrin Isotype Control (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCR3
CCR3 is a G protein-linked seven transmembrane chemokine receptor that preferentially binds eotaxin and eotaxin-2.
  • Entrez Gene IDs:
    1232 (Human); 12771 (Mouse); 117027 (Rat); 448802 (Canine)
  • Alternate Names:
    b-chemokine receptor; CC chemokine receptor 3; C-C chemokine receptor type 3; C-C CKR-3; CCR3; CCR-3; CD193 antigen; CD193; chemokine (C-C motif) receptor 3; CKR3CC-CKR-3CMKBR3CD193; eosinophil CC chemokine receptor 3; Eosinophil eotaxin receptor; MGC102841
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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Species
Applications
Sample Type
  1. Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver.
    Authors: Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH
    J. Immunol., 2010;184(6):2886-98.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. The Lupane-type Triterpene 30-Oxo-calenduladiol Is a CCR5 Antagonist with Anti-HIV-1 and Anti-chemotactic Activities.
    Authors: Barroso-Gonzalez J, El Jaber-Vazdekis N, Garcia-Exposito L, Machado JD, Zarate R, Ravelo AG, Estevez-Braun A, Valenzuela-Fernandez A
    J. Biol. Chem., 2009;284(24):16609-20.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Chemokine responsiveness of CD4+ CD25+ regulatory and CD4+ CD25- T cells from atopic and nonatopic donors.
    Authors: Ahern D, Lloyd CM, Robinson DS
    Allergy, 2009;64(8):1121-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Chemokine production and chemokine receptor expression by human glioma cells: role of CXCL10 in tumour cell proliferation.
    Authors: Maru SV, Holloway KA, Flynn G, Lancashire CL, Loughlin AJ, Male DK, Romero IA
    J. Neuroimmunol., 2008;199(1):35-45.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases.
    Authors: Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D
    J. Immunol., 2008;181(11):8053-67.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  6. Characterization of the human chemerin receptor--ChemR23/CMKLR1--as co-receptor for human and simian immunodeficiency virus infection, and identification of virus-binding receptor domains.
    Authors: Martensson UE, Fenyo EM, Olde B, Owman C
    Virology, 2006;355(1):6-17.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  7. Chemokine receptors in head and neck cancer: association with metastatic spread and regulation during chemotherapy.
    Authors: Muller A, Sonkoly E, Eulert C, Gerber PA, Kubitza R, Schirlau K, Franken-Kunkel P, Poremba C, Snyderman C, Klotz LO, Ruzicka T, Bier H, Zlotnik A, Whiteside TL, Homey B, Hoffmann TK
    Int. J. Cancer, 2006;118(9):2147-57.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  8. Chemokine receptor expression on human eosinophils from peripheral blood and bronchoalveolar lavage fluid after segmental antigen challenge.
    Authors: Liu LY, Jarjour NN, Busse WW, Kelly EA
    J. Allergy Clin. Immunol., 2003;112(3):556-62.
    Species: Human
    Sample Type: BALF
    Application: Flow
  9. Sequential involvement of CCR2 and CCR6 ligands for immature dendritic cell recruitment: possible role at inflamed epithelial surfaces.
    Authors: Vanbervliet B, Homey B, Durand I, Massacrier C, Ait-Yahia S, de Bouteiller O, Vicari A, Caux C
    Eur J Immunol, 2002;32(1):231-42.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A PE-conjugated Antibody

Ctrl IC006P 16  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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