Human Sirtuin 5/SIRT5 Biotinylated Antibody

Catalog #: BAF5914 Datasheet / COA / SDS

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Human Sirtuin 5/SIRT5 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Sirtuin 5/SIRT5 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) SIRT1, rhSIRT2, and rhSIRT6 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Sirtuin 5/SIRT5
Met33-Ser310
Accession # Q9NXA8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant human Sirtuin 5/SIRT5

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Sirtuin 5/SIRT5

Sirtuin 5 (SIRT5; SIR2-like protein 5) is a 28‑35 kDa class III member of the sirtuin protein family. It is found in the mitochondrial matrix and is involved in ammonia detoxification. In particular, it deacetylates carbamoyl phosphate synthetase 1/CPS1, thus activating it and allowing for ammonia condensation with bicarbonate and ATP. This is a crucial step in urea formation during prolonged fasting or the use of a high-protein diet. Human SIRT5 is 310 amino acids (aa) in length. It possesses a sertuin‑type deacetylase domain (aa 41‑309) that contains an NAD binding motif (Thr276:Glu277:Pro292). There are three potential isoform variants that show aa substitutions at the C‑terminus. One contains a 14 aa substitution for aa 286‑310, a second shows a 15 aa substitution for aa 287‑310, and the third shows a 17 aa substitution for aa 207‑310. Full-length human and mouse SIRT5 share 86% aa identity.

Long Name

Silent Mating Type Information Regulation 5 Homolog

Entrez Gene IDs

23408 (Human); 68346 (Mouse); 306840 (Rat)

Alternate Names

EC 3.5.1; EC 3.5.1.-; FLJ36950; NAD-dependent deacetylase sirtuin-5; silent mating type information regulation 2, S.cerevisiae, homolog 5; SIR2L5; sir2-like 5; SIR2-like protein 5; SIRT5; sirtuin (silent mating type information regulation 2 homolog) 5 (S. cerevisiae); sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 5; Sirtuin 5; sirtuin type 5