Human Slug Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
IC7408G
Human Slug Alexa Fluor® 488-conjugated Antibody in Data
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Human Slug Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Slug in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 666633
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Slug
Met1-His268
Accession # O43623
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of Slug antibody in HeLa Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Slug in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human Slug Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC7408G, filled histogram) or isotype control antibody (Catalog # IC003G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Slug

SLUG, also known as SNAI2, is a zinc-finger transcription factor. SLUG is a transcriptional repressor which may suppress E-cadherin transcription and be involved in an epithelial to mesenchymal transition (EMT).

Entrez Gene IDs
6591 (Human); 20583 (Mouse)
Alternate Names
MGC10182; Neural crest transcription factor Slug; Protein snail homolog 2; slug homolog, zinc finger protein (chicken); Slug; SLUGH; SLUGH1; SLUGzinc finger protein; SNAI2; snail 2; snail homolog 2 (Drosophila); SNAIL2; WS2D; zinc finger protein SNAI2

Product Datasheets

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