Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all Smad4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Smad4
Pro139-Asp332
Accession # Q13485

Specificity

Detects human Smad4 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) Smad1 is observed and less than 2% cross-reactivity with rhSmad5, rhSmad7, and rhSmad9 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Smad4 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Smad4

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Smad4

Smads are a family of intracellular proteins that transmit transforming growth factor beta (TGF-beta ) superfamily signals from the cell surface to the nucleus. Upon signal-induced phosphorylation, Smad subunits associate with the common-mediator subunit, Smad4. This heteromeric complex then translocates into the nucleus to exert transcriptional comodulator activity.

Long Name

Mothers Against DPP Homolog 4

Alternate Names

DPC4, MADH4

Entrez Gene IDs

4089 (Human)

Gene Symbol

SMAD4

UniProt

Q13485

Additional Smad4 Products

Product Documents for Human Smad4 Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Smad4 Biotinylated Antibody

For research use only

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FAQs

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