Human SMARCA5/SNF2H Alexa Fluor® 532-conjugated Antibody

Catalog #: AF6825X Datasheet / COA / SDS

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AF6825X-100UG

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Human SMARCA5/SNF2H Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human SMARCA5/SNF2H in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) SMARCA1 and rhSMARC4 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human SAMRCA5/SNF2H
Asp77-Val185
Accession # O60264

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: SMARCA5/SNF2H

SMARCA5 (Swi/SNF-related matrix-associated actin-dependent regulator of chromatin, subfamily A, member 5; also SNF2H) is a 130-135 kDa member of the SNF2/RAD54 helicase family of molecules. It is a ubiquitously expressed, chromatin remodeling ATPase that increases the accessibility of nucleosomal DNA to DNA-binding proteins. It apparently does so by sliding histone octamers over DNA. SMARCA5 is a component of four chromatin-remodeling complexes, including CHRAC, RSF, ACF and WICH. Human SMARCA5 is 1052 amino acids (aa) in length. It contains an N-terminal helicase domain (aa 192-357), a helicase superfamily C-terminal domain (aa 474-605), and two SANT DNA-binding domains (aa 840-892 and 943-1007). Over aa 77-185, human SMARCA5 shares 99% aa identity with mouse SMARCA5.

Long Name

SWI/SNF-related, Matrix-associated, Actin-dependent Regulator of Chromatin, Subfamily A, Member 5

Entrez Gene IDs

8467 (Human); 93762 (Mouse); 307766 (Rat)

Alternate Names

EC 3.6.1; EC 3.6.4.-; hISWI; hSNF2HSWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5; ISWI; SMARCA5; SNF2H; SNF2Hsucrose nonfermenting-like 5; subfamily a, member 5; Sucrose nonfermenting protein 2 homolog; SWI/SNF related, matrix associated, actin dependent regulator of chromatin; SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A member 5; WCRF135