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Human SMURF1 Alexa Fluor® 594-conjugated Antibody

Catalog #: FAB9507T Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB9507T-100UG

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SMURF1 Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human SMURF1 in direct ELISAs.
Source
Monoclonal Mouse IgG2b Clone # 530131
Purification
Protein A or G purified
Immunogen
E. coli-derived recombinant human SMURF1 
Met496-Glu757
Accession # Q9HCE7
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: SMURF1

Smurf-1 is a 757 amino acids E3 ubiquitin-protein ligase encoded by the SMURF1 gene. Smurf-1 ubiquitinates receptor regulated SMAD proteins acting as a negative regulator of BMP signaling pathway, and it has been shown to inhibit osteoblast differentiation by targeting Runx2, the master regulator of osteoblast differentiation, for degradation.

Long Name
SMAD Ubiquitination Regulatory Factor 1
Entrez Gene IDs
57154 (Human); 75788 (Mouse); 690516 (Rat)
Alternate Names
E3 ubiquitin ligase SMURF1; EC 6.3.2; EC 6.3.2.-; hSMURF1; KIAA1625; KIAA1625E3 ubiquitin-protein ligase SMURF1; SMAD specific E3 ubiquitin protein ligase 1; SMAD ubiquitination regulatory factor 1; Smad-specific E3 ubiquitin ligase 1; SMAD-specific E3 ubiquitin-protein ligase 1; SMURF1

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