Human SOX17 Alexa Fluor® 488-conjugated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human SOX17 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG1 Clone # 614013
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human SOX17
    Asp177-Val414
    Accession # Q9H6I2
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    8-25 µg/mL
    See below
  • Intracellular Staining by Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
SOX17 in BG01V Human Embryonic Stem Cells. SOX17 was detected in immersion fixed definitive endoderm-differentiated BG01V human embryonic stem cells using Mouse Anti-Human SOX17 Alexa Fluor® 488‑conjugated Monoclonal Antibody (green, left panel; Catalog # IC19241G) at 10 µg/mL for 3 hours at room temperature and counterstained with DAPI (blue, right panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Intracellular Staining by Flow Cytometry
Detection of SOX17 in Definitive Endoderm-differentiated BG01V Human Stem Cells by Flow Cytometry. Definitive endoderm-differentiated BG01V human embryonic stem cells were stained with Mouse Anti-Human SOX17 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC19241G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: SOX17
SOX17 is a member of the SOX family of transcription factors that bind DNA via a high mobility group (HMG) domain. SOX17 is suggested to play an important role in endoderm development (1, 2).
  • References:
    1. Kanai-Azuma, M. et al. (2002) Development 129:2367.
    2. Katoh, M. et al. (2002) Int. J. Mol. Med. 9:153.
  • Long Name:
    Transcription Factor SOX17
  • Entrez Gene IDs:
    64321 (Human)
  • Alternate Names:
    FLJ22252; SOX17; SRY (sex determining region Y)-box 17; SRY-related HMG-box transcription factor SOX17; transcription factor SOX-17; VUR3
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Alexa Fluor® 488-conjugated Antibody

Ctrl IC002G 3  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 5
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009 3
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007 2
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