Human SPINK1 Antibody Summary
Accession # P00995
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human SPINK1 by Western Blot. Western blot shows lysates of human pancreas tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human SPINK1 Monoclonal Antibody (Catalog # MAB7496) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SPINK1 at approximately 6 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human SPINK1 by Simple WesternTM. Simple Western lane view shows lysates of human pancreas tissue, loaded at 0.5 mg/mL. A specific band was detected for SPINK1 at approximately 6 kDa (as indicated) using 100 µg/mL of Mouse Anti-Human SPINK1 Monoclonal Antibody (Catalog # MAB7496). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
SPINK1 (Serine Protease Inhibitor Kazal-type 1; also TATI and PST1) is a 6-7 kDa secreted polypeptide initially identified as a tumor-derived trypsin inhibitor. It is widely expressed and found in cells diverse as pancreatic acinar cells, columnar cells of the stomach, renal collecting duct epithelium, and ureteric transitional plus breast epithelium. SPINK1 is known to be secreted with pancreatic zymogens, and apparently inactivates prematurely-activated trypsin, thus protecting the pancreas from trypsin-mediated enzyme activation. It also is reported to regulate cell migration and proliferation, the latter effect attributed to its structural resemblance to EGF and its ability to bind to activate the EGFR. Mature human SPINK1 is 56 amino acids (aa) in length (aa 24-79). It contains one Kazal-like domain (aa 26-79) that possesses a potential proteolytic cleavage site between Lys41-Ile42. An 11-12 kDa form in SDS-PAGE has been reported for SPINK1, possibly reflecting dimerization. Mature human SPINK1 shares 66% aa sequence identity with mouse Spink3, the mouse equivalent to human SPINK1.
Citation for Human SPINK1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Androgen deprivation upregulates SPINK1 expression and potentiates cellular plasticity in prostate cancer
Authors: R Tiwari, N Manzar, V Bhatia, A Yadav, MA Nengroo, D Datta, S Carskadon, N Gupta, M Sigouros, F Khani, M Poutanen, A Zoubeidi, H Beltran, N Palanisamy, B Ateeq
Nat Commun, 2020;11(1):384.
Sample Types: Cell Lysates
Applications: Western Blot
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