|Detection of Mouse IgG2B Primary Antibody by Flow Cytometry Human peripheral blood lymphocytes were stained with A) Mouse Anti-Human CD8 alpha Monoclonal Antibody (Catalog # MAB1509, Mouse IgG2B, filled histogram) or isotype control (Catalog # MAB0041, open histogram), or B) Mouse Anti-Human CD4 Monoclonal Antibody (Catalog # MAB3791, Mouse IgG2A, filled histogram) or isotype control (Catalog # MAB003, open histogram), followed by Rat Anti-Mouse IgG2B isotype-specific secondary antibody (Catalog # MAB0043) and then Allophycocyanin-conjugated Anti-Rat IgG SecondaryAntibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.|
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.