SPRED2 (Sprouty-related protein with an EVH1 domain-2) is a 47-53 kDa member of the SPRED family of regulatory molecules. In human, it is found in keratinocytes, intestinal columnar epithelium, and salivary duct epithelium. It inhibits receptor tyrosine kinase (RTK) signaling by blocking Ras activation of Raf. This is achieved by inducing the lysosomal degradation of RTKs following SPRED2 binding to NBR1. Human SPRED2 is 418 amino acids (aa) in length. It contains an N‑terminal WH1/EVH1 domain that is involved in ERK inhibition (aa 5-122), a central KBD domain that binds to the SCFR (aa 201-257), and a C-terminal Sprouty‑related domain that mediates homodimerization, and heterodimerization with SPRED1 (aa 308-416). SPRED is ubiquitinated, and undergoes phosphorylation on Ser, Thr and Tyr residues. There is one splice variant that shows a six aa substitution for aa 1-9. Over aa 117-297, human SPRED2 shares 84% aa identity with mouse SPRED2.
Human SPRED2 Alexa Fluor™ Plus 405‑conjugated Antibody
R&D Systems | Catalog # AF4819AFP405
Key Product Details
Species Reactivity
Applications
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Antibody Source
Product Specifications
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Isotype
Applications for Human SPRED2 Alexa Fluor™ Plus 405‑conjugated Antibody
Immunocytochemistry
Western Blot
Formulation, Preparation, and Storage
Formulation
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Stability & Storage
Background: SPRED2
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UniProt
Additional SPRED2 Products
Product Documents for Human SPRED2 Alexa Fluor™ Plus 405‑conjugated Antibody
Certificate of Analysis
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Product Specific Notices for Human SPRED2 Alexa Fluor™ Plus 405‑conjugated Antibody
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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Protocols
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- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars