Human ST2/IL-33R PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB5231P-100
FAB5231P-025
Detection of ST2/IL‑33 R4 in HUVEC Human Cells by Flow Cytometry.
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Product Details
Citations (6)
FAQs
Supplemental Products
Reviews (2)

Human ST2/IL-33R PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human ST2/IL-33 R in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) IL-1 RI, rhIL-1 RII, rhIL-18 RI, and rhST-7 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human ST2/IL-33 R
Lys19-Phe328
Accession # BAA02233
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of ST2/IL‑33 R4 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Goat Anti-Human ST2/IL-33 R PE-conjugated Polyclonal Antibody (Catalog # FAB5231P, filled histogram) or isotype control antibody (IC108P, open histogram). Staining was performed using our Staining Membrane-associated Proteins protocol.

Flow Cytometry Detection of ST2/IL-33 R4 antibody in KG-1a Human Cell Line antibody by Flow Cytometry. View Larger

Detection of ST2/IL‑33 R4 in KG‑1a Human Cell Line by Flow Cytometry. KG-1a human acute myelogenous leukemia cell line was stained with Goat Anti-Human ST2/IL-33 R PE-conjugated Polyclonal Antibody (Catalog # FAB5231P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: ST2/IL-33R

ST2, also known as IL-33R, IL-1 R4 and T1, is an Interleukin-1 receptor family glycoprotein that contributes to Th2 immune responses (1, 2). Human ST2 consists of a 310 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 21 aa transmembrane segment, and a 207 aa cytoplasmic domain with an intracellular TIR domain (3, 4). Alternate splicing of the 120 kDa human ST2 generates a soluble 60 kDa isoform that lacks the transmembrane and cytoplasmic regions as well as an isoform that additionally lacks the third Ig‑like domain (4). Within the ECD, human ST2 shares 68% and 64% aa sequence identity with mouse and rat ST2, respectively. ST2 is expressed on the surface of mast cells, activated Th2 cells, macrophages, and cardiac myocytes (5‑8). It binds IL-33, a cytokine that is upregulated by inflammation or mechanical strain in smooth muscle cells, airway epithelia, keratinocytes, and cardiac fibroblasts (5, 9). IL-33 binding induces the association of ST2 with IL-1R AcP, a shared signaling subunit that also associates with IL-1 RI and IL-1 R rp2 (1, 10, 11). In macrophages, ST2 interferes with signaling from IL-1 RI and TLR4 by sequestering the adaptor proteins MyD88 and Mal (7). In addition to its role in promoting mast cell and Th2 dependent inflammation, ST2 activation enhances antigen induced hypernociception and protects from atherosclerosis and cardiac hypertrophy (5, 12‑14). The soluble ST2 isoform is released by activated Th2 cells and strained cardiac myocytes and is elevated in the serum in allergic asthma (6, 8, 15). Soluble ST2 functions as a decoy receptor that blocks IL‑33's ability to signal through transmembrane ST2 (10, 13‑15).

References
  1. Barksby, H.E. et al. (2007) Clin. Exp. Immunol. 149:217.
  2. Gadina, M. and C.A. Jefferies (2007) Science STKE 2007:pe31.
  3. Tominaga, S. et al. (1992) Biochim. Biophys. Acta 1171:215.
  4. Li, H. et al. (2000) Genomics 67:284.
  5. Schmitz, J. et al. (2005) Immunity 23:479.
  6. Lecart, S. et al. (2002) Eur. J. Immunol. 32:2979.
  7. Brint, E.K. et al. (2004) Nat. Immunol. 5:373.
  8. Weinberg, E.O. et al. (2002) Circulation 106:2961.
  9. Sanada S. et al. (2007) J. Clin. Invest. 117:1538.
  10. Palmer, G. et al. (2008) Cytokine 42:358.
  11. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
  12. Allakhverdi, Z. et al. (2007) J. Immunol. 179:2051.
  13. Verri Jr., W.A. et al. (2008) Proc. Natl. Acad. Sci. 105:2723.
  14. Miller, A.M. et al. (2008) J. Exp. Med. 205:339.
  15. Hayakawa, H. et al. (2007) J. Biol. Chem. 282:26369.
Long Name
Interleukin 33 Receptor
Entrez Gene IDs
9173 (Human); 17082 (Mouse)
Alternate Names
DER4; DER4ST2growth stimulation-expressed; Fit-1; IL-1 R4; IL1R4; IL-1R4; IL-1RL1; IL-33 R; IL33R; IL-33R; interleukin 1 receptor-like 1; interleukin 1 receptor-related protein; interleukin-1 receptor-like 1; Ly84; MGC32623; Protein ST2; ST2; ST2L; ST2V; T1; T1homolog of mouse growth stimulation-expressed

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Citations for Human ST2/IL-33R PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Organoids capture tissue-specific innate lymphoid cell development in mice and humans
    Authors: GM Jowett, E Read, LB Roberts, D Coman, M Vilà Gonzá, T Zabinski, U Niazi, R Reis, TJ Trieu, D Danovi, E Gentleman, L Vallier, MA Curtis, GM Lord, JF Neves
    Cell Reports, 2022;40(9):111281.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Interleukin-33 activates group 2 innate lymphoid cell expansion and modulates endometriosis
    Authors: JE Miller, H Lingegowda, LK Symons, O Bougie, SL Young, BA Lessey, M Koti, C Tayade
    JCI Insight, 2021;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Acid Stripping of Surface IgE Antibodies Bound to Fc&epsilonRI is Unsuitable for the Functional Assays that Require Long-Term Culture of Basophils and Entire Removal of Surface IgE
    Authors: C Galeotti, A Karnam, M Das, SV Kaveri, J Bayry
    Int J Mol Sci, 2020;21(2):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Hallmarks of primate lentiviral immunodeficiency infection recapitulate loss of innate lymphoid cells
    Authors: JC Mudd, K Busman-Sah, SR DiNapoli, S Lai, V Sheik, A Lisco, C Deleage, B Richardson, DJ Palesch, M Paiardini, M Cameron, I Sereti, RK Reeves, JD Estes, JM Brenchley
    Nat Commun, 2018;9(1):3967.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. SP and IL-33 together markedly enhance TNF synthesis and secretion from human mast cells mediated by the interaction of their receptors
    Authors: A Taracanova, M Alevizos, A Karagkouni, Z Weng, E Norwitz, P Conti, SE Leeman, TC Theoharide
    Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. BCR-ABL-induced deregulation of the IL-33/ST2 pathway in CD34+ progenitors from chronic myeloid leukemia patients.
    Authors: Levescot A, Flamant S, Basbous S, Jacomet F, Feraud O, Anne Bourgeois E, Bonnet M, Giraud C, Roy L, Barra A, Chomel J, Turhan A, Guilhot F, Girard J, Gombert J, Herbelin A
    Cancer Res, 2014;74(10):2669-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Human ST2/IL-33R PE-conjugated Antibody
By Anonymous on 10/13/2021
Application: Flow Sample Tested: LAD2 human mast cell line Species: Human

Human ST2/IL-33 R PE-conjugated Antibody
By Anonymous on 01/11/2018
Application: Flow Sample Tested: Blood mononuclear cells (PBMCs) Species: Human